Implications of rewiring bacterial quorum sensing
Implications of rewiring bacterial quorum sensing
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DOI：
10.1128/aem.01688-07
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发表时间：
2008-01-01
影响因子：
4.4
通讯作者：
Arnold, Frances H.
中科院分区：
文献类型：
--
作者：
Haseltine, Eric L.;Arnold, Frances H.
细菌利用群体感应，这是细胞间交流的一种形式，来感知种群密度的变化，并相应地调节基因表达。这项工作研究了一个群体感应模块，来自海洋细菌费氏弧菌的勒克斯电路的重新布线。稳态实验表明，重新布线该模块的网络结构可以产生数学模型预测的分级、阈值和双稳基因表达。实验还表明，天然的勒克斯操纵子与阈值反应最一致，而不是双稳反应。每个重新连接的网络在生物相关的条件下都产生了功能良好的种群传感器，这表明这个操纵子特别强大。这些发现(I)允许预测细菌病原体中群体感应操纵子的行为，(Ii)促进合成基因电路的正向工程。
Bacteria employ quorum sensing, a form of cell-cell communication, to sense changes in population density and regulate gene expression accordingly. This work investigated the rewiring of one quorum-sensing module, the lux circuit from the marine bacterium Vibrio fischeri. Steady-state experiments demonstrate that rewiring the network architecture of this module can yield graded, threshold, and bistable gene expression as predicted by a mathematical model. The experiments also show that the native lux operon is most consistent with a threshold, as opposed to a bistable, response. Each of the rewired networks yielded functional population sensors at biologically relevant conditions, suggesting that this operon is particularly robust. These findings (i) permit prediction of the behaviors of quorum-sensing operons in bacterial pathogens and (ii) facilitate forward engineering of synthetic gene circuits.