A fixed cytometer chip for identification of cell populations and real-time monitoring of single-cell apoptosis under gradient UV radiation
A fixed cytometer chip for identification of cell populations and real-time monitoring of single-cell apoptosis under gradient UV radiation
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梯度紫外辐射下细胞群鉴定和单细胞凋亡实时监测的固定式细胞计数芯片
DOI：
10.1007/s10404-019-2244-8
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发表时间：
2019-05
影响因子：
2.8
通讯作者：
Liu Peifeng
中科院分区：
文献类型：
--
作者：
Xia Anyue;Gan Mingzhe;Xu Huan;Zhang Yiheng;Wang D;an;Du Jing;Sun Qian;Jiang Jiana;Luo Dan;Cui Jinhui;Liu Peifeng
细胞计数是确定细胞群体和形态的基本方法。流式细胞术和血细胞计数是细胞计数技术中最常用的两种方法。然而，流式细胞术需要庞大而昂贵的设备以及专业的操作，而血细胞计数术因其简单的功能而受到限制。这两种方法都不适合实时监测单个细胞的形态变化。在这里，我们开发了一种固定的流式细胞仪芯片，具有两种功能模式，用于识别细胞群（I模式）和实时监测单细胞形态变化（M模式）。在I模式下，采用固定式流式细胞仪芯片检测细胞群，结果与血细胞计数和流式细胞仪检测结果一致。此外，通过测量单个细胞的双色荧光强度进一步精确地鉴定细胞群体，这与流式细胞术中的双参数分析一致。在M模式下，该芯片被应用于实时监测梯度紫外辐射下的单细胞凋亡，所述梯度紫外辐射由一种新型的阶梯状紫外屏蔽物产生。通过延时成像实时监测大量单细胞的动态凋亡形态。此外，在60 mm × 30 mm芯片上集成了8个并行通道，可实现可扩展的单细胞捕获和分析能力。这种固定式流式细胞仪芯片具有双功能、易于操作、通用性和可扩展性。
Cytometry is a basic method to determine cell populations and morphology. Flow cytometry and hemocytometry are the two most common methods among cytometric technologies. However, flow cytometry needs bulky and expensive equipment as well as professional operations, while hemocytometry is limited by its simple function. Both of them are not suitable for real-time monitoring of the morphological changes of single cells. Here, we developed a fixed cytometer chip with two functional modes for both identification of cell populations (I-mode) and real-time monitoring of single-cell morphological changes (M-mode). In I-mode, the fixed cytometer chip was employed to evaluate the cell populations, the results were in accordance with those from the hemocytometer counting and flow cytometry. Besides that, the cell populations were further precisely identified by measuring two-color fluorescence intensities of single cells, which were consistent with the dual parameter analysis in flow cytometry. In M-mode, the chip was applied to real-time monitoring of the single-cell apoptosis under gradient UV radiation, generated by a novel stair-like UV shield. The dynamic apoptotic morphologies of a large number of single cells were monitored in real-time by time-lapse imaging. In addition, we integrated eight parallel channels on a 60 mm × 30 mm chip, and the chip could achieve scalable single-cell capture and analysis capability. This fixed cytometer chip is bifunctional, easy-to-handle, universal, and scalable.
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影响因子：
13.8
作者：
W. Salmhofer;H. Soyer;P. Wolf;D. Födinger;S. Hödl;H. Kerl
通讯作者：
W. Salmhofer;H. Soyer;P. Wolf;D. Födinger;S. Hödl;H. Kerl
影响因子：
6.1
作者：
Cho SH;Chen CH;Tsai FS;Godin JM;Lo YH
通讯作者：
Lo YH
影响因子：
3.4
作者：
Xu J;Chen S;Wang D;Jiang Y;Hao M;Du G;Ba D;Lin Q;Mei Q;Ning Y;Su D;Liu K
通讯作者：
Liu K
影响因子：
3.9
作者：
Xin Cui;H. Yip;Qian Zhu;Cheng-Wei Yang;Raymond H. W. Lam
通讯作者：
Xin Cui;H. Yip;Qian Zhu;Cheng-Wei Yang;Raymond H. W. Lam
影响因子：
37.3
作者：
Sarkar S;Rajput S;Tripathi AK;Mandal M
通讯作者：
Mandal M