Requirement for B cell linker protein (BLNK) in B cell development
Requirement for B cell linker protein (BLNK) in B cell development
复制标题
DOI：
10.1126/science.286.5446.1949
复制
发表时间：
1999-12-03
期刊：
影响因子：
56.9
通讯作者：
Chan, AC
中科院分区：
文献类型：
--
作者：
Pappu, R;Cheng, AM;Chan, AC
连接蛋白充当分子支架，将酶与底物定位。在 B 细胞中，B 细胞连接蛋白 (BLNK) 将 B 细胞受体 (BCR) 激活的 Syk 激酶与磷酸肌醇和丝裂原激活的激酶途径连接起来。为了检查 BLNK 的体内作用，产生了 BLNK 缺陷的小鼠。 BLNK-/-小鼠中的B细胞发育在从B220(+)CD43(+)祖B细胞向B220(+)CD43(-)前体B细胞的转变过程中被阻断。在外周血中仅检测到一小部分免疫球蛋白 M++ (IgM(++))，但未检测到成熟的 IgM(lo)lgD(hi)、B 细胞。因此，BLNK 是 BCR 信号通路的重要组成部分，是促进 B 细胞发育所必需的。
Linker proteins function as molecular scaffolds to Localize enzymes with substrates. In B cells, B cell Linker protein (BLNK) Links the B cell receptor (BCR)activated Syk kinase to the phosphoinositide and mitogen-activated kinase pathways. To examine the in vivo role of BLNK, mice deficient in BLNK were generated. B cell development in BLNK-/- mice was blocked at the transition from B220(+) CD43(+) progenitor B to B220(+) CD43(-) precursor B cells. Only a small percentage of immunoglobulin M++ (IgM(++)), but not mature IgM(lo)lgD(hi), B cells were detected in the periphery. Hence, BLNK is an essential component of BCR signaling pathways and is required to promote B cell development.