Echinococcus granulosus Infection Results in an Increase in Eisenbergiella and Parabacteroides Genera in the Gut of Mice
Echinococcus granulosus Infection Results in an Increase in Eisenbergiella and Parabacteroides Genera in the Gut of Mice
复制标题
细粒棘球绦虫感染导致小鼠肠道内艾森氏菌属和副杆菌属增加
DOI：
10.3389/fmicb.2018.02890
复制
发表时间：
2018-11-29
影响因子：
5.2
通讯作者：
McManus, Donald P.
中科院分区：
文献类型：
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作者：
Bao, Jianling;Zheng, Huajun;McManus, Donald P.
囊性棘球蚴病是一种由细粒棘球蚴引起的慢性传染病。为确认感染是否对肠道菌群有影响，本研究采用BALB/c小鼠感染颗粒棘球绦虫微囊建立小鼠感染模型。感染4个月后，收集粪便样本，对16S rRNA基因的高变区进行高通量测序。序列分析显示，共有13353个otu，其中只有40.6%的otu具有属参考信息，101个otu在感染小鼠中显著增加。生物信息学分析表明，共同核心微生物群在家族水平上没有显著变化。在属水平上，感染小鼠体内富集了2个属（Eisenbergiella和Parabacteroides） （P-AMOVA < 0.05）。功能分析表明，与未感染组相比，颗粒棘球绦虫感染组有7条通路发生改变。Spearman相关分析显示，IgG、IgG1和IgG2a与9个主要属有较强的相关性。颗粒芽胞杆菌囊肿感染可改变肠道菌群，这可能与代谢途径有关。
Cystic echinococcosis (CE) is a chronic infectious disease caused by Echinococcus granulosus. To confirm whether the infection impacts on the gut microbiota, we established a mouse model of E. granulosus infection in this study whereby BALB/c mice were infected with micro-cysts of E granulosus. After 4 months of infection, fecal samples were collected for high-throughput sequencing of the hypervariable regions of the 16S rRNA gene. Sequence analysis revealed a total of 13,353 operational taxonomic units (OTUs) with only 40.6% of the OTUs having genera reference information and 101 of the OTUs were significantly increased in infected mice. Bioinformatics analysis showed that the common core microbiota were not significantly changed at family level. However, two genera (Eisenbergiella and Parabacteroides) were enriched in the infected mice (P-AMOVA < 0.05) at genus level. Functional analysis indicated that seven pathways were altered in the E. granulosus Infection Group compared with the Uninfected Group. Spearman correlation analysis showed strong correlations of IgG, IgG1 and IgG2a with nine major genera. E. granulosus cyst infection may change the gut microbiota which may be associated with metabolic pathways.