Activation of retinal ganglion cells in wild-type and rd1 mice through electrical stimulation of the retinal neural network
Activation of retinal ganglion cells in wild-type and rd1 mice through electrical stimulation of the retinal neural network
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DOI：
10.1016/j.visres.2008.04.016
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发表时间：
2008-06-01
期刊：
影响因子：
1.8
通讯作者：
Rizzo, Joseph F., III
中科院分区：
文献类型：
--
作者：
Jensen, Ralph J.;Rizzo, Joseph F., III
我们比较了野生型和Rd1小鼠视网膜细胞外记录的视网膜神经节细胞(RGC)对视网膜神经网络电刺激的阈值和反应特性。用直径为400微米的视网膜下电极施加双相电流脉冲(1ms/相)，在体外刺激视网膜。在野生型和Rd1 RGCs中均观察到三种类型的反应。在施加电刺激后，I型细胞在20INS内诱发单次尖峰放电，II型细胞诱发潜伏期大于37ms的单次尖峰放电，III型细胞诱发两次或偶尔三次尖峰放电。在研究的所有年龄，从出生后25天(P)到出生后186天，RGCs的阈值在高个子鼠中总体上一直较高。野生型和Rd1小鼠的中位数阈值分别为14和50 mA。我们认为，光感受器降低视网膜节细胞的激活阈值，而感受器后神经元决定视网膜节细胞对电刺激的反应特性。爱思唯尔有限公司出版。
We compared the thresholds and response properties of extracellularly recorded retinal ganglion cells (RGCs) in wild-type and rd1 mouse retinas to electrical stimulation of the retinal neural network. Retinas were stimulated in vitro with biphasic current pulses (1 ms/phase) applied with a 400-mu m diameter, subretinal electrode. Three types of responses were observed in both wild-type and rd1 RGCs. Type I cells elicited a single burst of spikes within 20 ins following application of the electrical stimulus, type II cells elicited a single burst of spikes with a latency greater than 37 ms, and type III cells elicited two and occasionally three bursts of spikes. For all ages examined, ranging from postnatal day (P) 25 to P186, the thresholds of RGCs were overall consistently higher in tall mice. Median threshold values were 14 and 50 mu A in wild-type and rd1 mice, respectively. We propose that photoreceptors lower the thresholds for activation of RGCs whereas postreceptoral neurons determine the response properties ofRGCs to electrical stimuli. Published by Elsevier Ltd.