Modulation of haematopoetic system and antioxidant enzymes by Emblica officinalis Gaertn and its protective role against γ-radiation induced damages in mice
Modulation of haematopoetic system and antioxidant enzymes by Emblica officinalis Gaertn and its protective role against γ-radiation induced damages in mice
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DOI：
10.1269/jrr.45.549
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发表时间：
2004-12-01
影响因子：
2
通讯作者：
Kuttan, R
中科院分区：
文献类型：
--
作者：
Kumar, KB;Sabu, MC;Kuttan, R
用瑞士白化病小鼠研究余甘子果肉的辐射防护作用。在照射前用2.5g/kg B.wt的余甘子处理小鼠连续10天，并在最后一次剂量后暴露于700拉德（7 Gy）的单剂量辐射。一组在照射后继续给予余甘子15天。观察全身照射后白细胞总数、骨髓细胞活力和血红蛋白的变化。Emblica的管理显着增加这些水平，这是降低辐射。在照射后不同时间点处死动物，测定血液中抗氧化酶过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）和谷胱甘肽-S-转移酶（GST）的活性以及谷胱甘肽的水平。通过测定组织脂质过氧化物水平，研究了全身照射后细胞膜的损伤。给药余甘子显著增强了血液中各种抗氧化酶和GST以及谷胱甘肽系统的活性。Emblica治疗还降低了血清中脂质过氧化物的升高水平。数据清楚地表明，提取物大大减少了辐射的生物效应。余甘子提取物可用于减少治疗放射期间产生的副作用。
The radio protective effect of the fruit pulp of Emblica officinalis Gaertn (Emblica) was studied in adult Swiss albino mice. Mice were treated with 2.5g/kg b.wt of Emblica for 10 consecutive days before irradiation and exposed to a single dose of 700 rads (7Gy) of radiation after the last dose. One group was given Emblica continuously for another 15 days after irradiation. Changes in the total leukocyte count, bone marrow viability and hemoglobin were studied after whole body irradiation. Administration of Emblica significantly increased these levels, which were lowered by irradiation. Animals were sacrificed at various time points after irradiation and the activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), and glutathione-S-transferase (GST), and levels of glutathione were assayed in the blood. The damage to the cell membrane after whole body irradiation was studied by measuring the tissue lipid peroxides levels. Administration of Emblica significantly enhanced the activity of the various antioxidant enzymes and GST as well as glutathione system in the blood. Treatment with Emblica also lowered the elevated levels of lipid peroxides in the serum. The data clearly indicated that the extract significantly reduced the bioeffects of radiation. Emblica extract may be useful in reducing the side effects produced during therapeutic radiation.