The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans
The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans
复制标题
DOI：
10.1210/jc.87.6.2988
复制
发表时间：
2002-06-01
影响因子：
5.8
通讯作者：
Korbonits, M
中科院分区：
文献类型：
--
作者：
Gnanapavan, S;Kola, B;Korbonits, M
胃饥饿素是一种新型的生长激素释放肽，最初在大鼠胃中被鉴定为生长激素促分泌素受体（GHS - R1a）的内源性配体。胃饥饿素参与生长激素释放的调节，但最近有人提出，胃饥饿素可能具有其他作用，包括对食欲、碳水化合物代谢、心脏、肾脏、胰腺、性腺和细胞增殖的影响。利用经典的和实时逆转录聚合酶链反应，研究了胃饥饿素、其功能性受体（1a型）以及未剪接的无功能的1b型生长激素促分泌素受体mRNA在人体各种组织中的分布。GHS - R1a主要在垂体中表达，在甲状腺、胰腺、脾脏、心肌和肾上腺中的表达水平要低得多。相比之下，胃饥饿素在胃、肠道的其他部位以及实际上在所研究的所有组织（肾上腺、心房、乳腺、口腔黏膜、食管、输卵管、脂肪组织、胆囊、人淋巴细胞、回肠、肾脏、左结肠、肝脏、肺、淋巴结、肌肉、心肌、卵巢、胰腺、垂体、胎盘、前列腺、右结肠、皮肤、脾脏、睾丸、甲状腺和静脉）中都有发现。GHS - R1b的表达在所有研究的组织中也很广泛。胃饥饿素在组织中广泛分布的意义仍有待确定。这些数据表明，胃饥饿素可能通过内分泌和非内分泌组织中不同的、部分尚未确定的GHS - R亚型产生广泛的生理效应。
Ghrelin is a novel growth hormone-releasing peptide, originally identified in the rat stomach as the endogenous ligand for the growth hormone secretagogue-receptor (GHS-R1a). Ghrelin is involved in the regulation of GH release, but it has recently been suggested that ghrelin may have other actions, including effects on appetite, carbohydrate metabolism, heart, kidney, pancreas, gonads, and cell proliferation. The distribution of ghrelin, its functional receptor (type la) and the unspliced, nonfunctional GHS-R type 1b mRNA expression was investigated in various human tissues using classical and real-time reverse transcription and polymerase chain reaction. GHS-R1a was predominantly expressed in the pituitary and at much lower levels in the thyroid gland, pancreas, spleen, myocardium and adrenal gland. In contrast, ghrelin was found in the stomach, other parts of the gut and, indeed, in all the tissues studied (adrenal gland, atrium, breast, buccal mucosa, esophagus, Fallopian tube, fat tissue, gall bladder, human lymphocytes, ileum, kidney, left colon, liver, lung, lymph node, muscle, muscle, myocardium, ovary, pancreas, pituitary, placenta, prostate, right colon, skin, spleen, testis, thyroid, and vein). GHS-R1b expression was also widespread in all tissues studied. The significance of the widespread tissue distribution of ghrelin remains to be determined. These data suggest that ghrelin might have widespread physiological effects via different, partly unidentified, subtypes of the GHS-R in endocrine and non-endocrine tissues.