A new allele of interferon-α17 gene encoding IFN-α17b is the major variant in human population
A new allele of interferon-α17 gene encoding IFN-α17b is the major variant in human population
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DOI：
10.1089/jir.1998.18.469
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发表时间：
1998-07-01
影响因子：
2.3
通讯作者：
Liao, MJ
中科院分区：
文献类型：
--
作者：
Hussain, M;Tan, T;Liao, MJ
已报道了13个干扰素（IFN）-α功能基因。其中，许多基因具有等位基因成员（变体）。在IFN-α 17的情况下，已知五种变体，IFN-α 17 a、IFN-α 17 b、IFN-α 17 c、IFN-α 17 d和IFN-α T。这些变体通过编码区的碱基变化而彼此不同，从而导致氨基酸序列的差异。我们已经开发了用于使用聚合酶链反应（PCR）扩增IFN-α 17基因的寡核苷酸引物。从超过28，000名正常健康个体和四种细胞系中获得的基因组DNA用作PCR中的模板以扩增IFN-α 17基因序列。通过限制性内切酶消化和DNA测序分析所得PCR产物以鉴定变体序列的存在。结果显示，在所检查的群体的基因组DNA中，IFN-α 17的新变体与另一种变体（可能是IFN-α 17 c（类似于30%））一起大量存在（类似于70%）沿着。这种新的变体，其蛋白质产物与IFN-α 17 b相同，与IFN-α 17 b的基因不同之处在于点突变。我们将其命名为IFN-α 17 b '，这是在U-937、KG-1和EB-3细胞系中发现的唯一变体。Namalwa细胞具有等比例的IFN-α 17 b '和可能的IFN-α 17 c。
Thirteen interferon (IFN)-alpha functional genes have been reported. Among these, a number of genes have allelic members (variants). In the case of IFN-alpha 17, five variants, IFN-alpha 17a, IFN-alpha 17b, IFN-alpha 17c, IFN-alpha 17d, and IFN-alpha T, are known. The variants differ from each other by base changes in the coding region, leading to differences in amino acid sequences. We have developed oligonucleotide primers for amplification of IFN-alpha 17 gene(s) using polymerase chain reaction (PCR). Genomic DNA, obtained from over 28,000 normal healthy individuals and from four cell lines, were used as templates in PCR to amplify the IFN-alpha 17 gene sequences. The resulting PCR products were analyzed by restriction endonuclease digestion and DNA sequencing to identify the presence of variant sequences. The results show that a new variant of IFN-alpha 17 is abundantly present (similar to 70%) along with another variant, possibly IFN-alpha 17c (similar to 30%), in the genomic DNA of the population examined. This new variant, the protein product of which is identical to IFN-alpha 17b, differs from the gene for IFN-alpha 17b by a point mutation. We have named it IFN-alpha 17b', which is the only variant found in U-937, KG-1, and EB-3 cell lines. Namalwa cells have IFN-alpha 17b' and, possibly, IFN-alpha 17c in equal proportions.