Proteomic analysis provides an insight into the molecular mechanism of development and flowering in Lycoris radiata
Proteomic analysis provides an insight into the molecular mechanism of development and flowering in Lycoris radiata
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DOI：
10.1007/s11738-020-03179-w
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发表时间：
2021-01-02
影响因子：
2.6
通讯作者：
Cai, Junhuo
中科院分区：
文献类型：
--
作者：
Jiang, Xueru;Chen, Huiting;Cai, Junhuo
石蒜是中国原产的一种重要的观赏、药用植物。它的生产和繁殖阶段是分开的。然而，在蛋白质组水平上，控制这一独特的发育和开花过程的分子机制仍然不清楚。本研究采用无标记定量蛋白质组学方法，研究了放射草鳞茎在休眠(Dor)、开花(Flo)、出叶(LO)、快速展叶(RLE)、叶片成熟(LMA)和叶片枯萎(LWI)六个发育阶段的蛋白质组动态变化。在鳞茎顶端共鉴定出2,090个蛋白质，每一对阶段都有242个(Flo与Dor)、82个(LO与Flo)、156个(RLE与LO)、76个(LMA与RLE)、438个(LWI与LMA)和164个(Dor与LWI)差异表达蛋白(DEPS)。基因本体论(GO)和途径浓缩分析表明，Flo和Dor的DEPS主要参与“能量储备代谢过程”、“细胞多糖代谢过程”和“细胞葡聚糖代谢过程”，而Lo和Flo、RLe和Lo的DEPS主要参与“细胞内类固醇激素受体信号通路的负调控”。加权基因共表达网络分析表明，冬眠期和开花期存在温度刺激、逆境反应和免疫反应，热休克蛋白83、热休克同源蛋白2类异构体X1和超氧化物歧化酶[铜-锌]4A等蛋白质参与了这些反应。此外，40S核糖体蛋白和细胞分裂周期5类蛋白参与了叶片阶段的蛋白质合成、细胞分裂和细胞扩张。这些结果有助于我们更好地了解辐射石蒜发育和开花的分子机制，并为了解石蒜属其他植物的发育和逆境反应所涉及的蛋白质提供有价值的信息。
Lycoris radiata Herb is an important ornamental and medicinal plant native in China. Its productive and reproductive phases are separate. However, the molecular mechanisms governing this unique process of development and flowering are still unclear at the proteome level. In the present study, we used a label-free quantitative proteomic approach to investigate the dynamic changes in the proteome of the bulb tips at six developmental stages in L. radiata, including dormancy (Dor), flowering (Flo), leafing out (LO), rapid leaf extension (RLE), leaf maturity (LMa) and leaf withering (LWi). A total of 2,090 proteins were identified in bulb tips, and each pair of the stages shared 242 (Flo vs. Dor), 82 (LO vs. Flo), 156 (RLE vs. LO), 76 (LMa vs. RLE), 438 (LWi vs. LMa) and 164 (Dor vs. LWi) differentially expressed proteins (DEPs). Analysis of Gene Ontology (GO) and pathway enrichment revealed that the DEPs of Flo vs. Dor were mainly involved in "energy reserve metabolic process", "cellular polysaccharide metabolic process" and "cellular glucan metabolic process", whereas the DEPs in LO vs. Flo and RLE vs. LO were mainly involved in "negative regulation of intracellular steroid hormone receptor signaling pathway". Weighted gene coexpression network analysis showed that there were temperature stimuli, stress responses and immune responses at the dormancy and flowering stages; several proteins, including heat shock protein 83, heat shock cognate 70 kDa protein 2-like isoform X1 and superoxide dismutase [Cu-Zn] 4A, can contribute to these responses. Additionally, 40S ribosomal protein and cell division cycle 5-like protein participated in protein synthesis, cell division and cell expansion during the leaf phase. These results can help us better understand the molecular mechanism of L. radiata development and flowering and provide valuable information about the proteins involved in the development and stress response in other plants of the Lycoris genus.