The translocation of fullerenic nanoparticles into lysosome via the pathway of clathrin-mediated endocytosis
The translocation of fullerenic nanoparticles into lysosome via the pathway of clathrin-mediated endocytosis
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DOI：
10.1088/0957-4484/19/14/145102
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发表时间：
2008-04-09
期刊：
影响因子：
3.5
通讯作者：
Zhang, Yingge
中科院分区：
文献类型：
--
作者：
Li, Wei;Chen, Chunying;Zhang, Yingge
制造的富勒烯纳米颗粒很容易进入细胞，因此已迅速发展为生物医学用途。然而，通常不知道纳米颗粒在穿过细胞膜时经历哪种途径以及它们定位于细胞内区室的何处。在此，我们使用显微成像和生物技术来探索[C-60（C（COOH）（2））（2）]（n）纳米颗粒穿过细胞膜的过程及其在3 T3 L1和RH-35活细胞中的胞内移位。富勒烯纳米颗粒被细胞迅速内化，然后以点状定位的方式进入细胞质。在进入细胞后，它们与溶酶体样囊泡同步。[C-60（C（COOH）（2））（2）]（n）纳米粒子主要通过内吞作用进入细胞，并具有时间、温度和能量依赖性。发现[C-60（C（COOH）（2））（2）]（n）纳米颗粒的细胞摄取是网格蛋白介导的，而不是小窝介导的内吞作用。内吞机制和亚细胞靶点定位为更好地理解和预测富勒烯纳米颗粒在细胞内的生物医学功能提供了关键信息。
Manufactured fullerene nanoparticles easily enter into cells and hence have been rapidly developed for biomedical uses. However, it is generally unknown which route the nanoparticles undergo when crossing cell membranes and where they localize to the intracellular compartments. Herein we have used both microscopic imaging and biological techniques to explore the processes of [C-60(C(COOH)(2))(2)](n) nanoparticles across cellular membranes and their intracellular translocation in 3T3 L1 and RH-35 living cells. The fullerene nanoparticles are quickly internalized by the cells and then routed to the cytoplasm with punctate localization. Upon entering the cell, they are synchronized to lysosome-like vesicles. The [C-60(C(COOH)(2))(2)](n) nanoparticles entering cells are mainly via endocytosis with time-, temperature-and energy-dependent manners. The cellular uptake of [C-60(C(COOH)(2))(2)](n) nanoparticles was found to be clathrin-mediated but not caveolae-mediated endocytosis. The endocytosis mechanism and the subcellular target location provide key information for the better understanding and predicting of the biomedical function of fullerene nanoparticles inside cells.