Effects of anesthesia (tricaine methanesulfonate, MS222) on liver biotransformation in rainbow trout (Oncorhynchus mykiss)
Effects of anesthesia (tricaine methanesulfonate, MS222) on liver biotransformation in rainbow trout (Oncorhynchus mykiss)
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DOI：
10.1016/s0166-445x(03)00051-1
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发表时间：
2003-07-16
影响因子：
4.5
通讯作者：
Schmieder, PK
中科院分区：
文献类型：
--
作者：
Kolanczyk, RC;Fitzsimmons, PN;Schmieder, PK
采用微粒体模型研究了三卡因甲烷磺酸盐（MS222）对虹鳟鱼肝脏生物转化速率的影响；麻药一种体外制剂，可以使用麻醉剂或不使用麻醉剂对两组虹鳟鱼微粒体进行了实验；一个代表体内或手术三卡因暴露，另一个代表体外组织/器官采集三卡因暴露。以苯酚为底物进行微粒体培养，通过监测对苯二酚（HQ）、儿茶酚（CAT）和苯葡萄糖醛酸盐（PG）的产生，评估三卡因对ⅰ期（环羟基化）和H期（葡萄糖醛酸盐化）肝脏生物转化的影响。使用100mg /l的三卡因进行2小时的手术麻醉，有或没有24小时的恢复，对苯酚（I期和II期）的生物转化率没有显著影响（P小于或等于0.05）；在分离器官/组织收集中，暴露于300 mg/l三卡因5分钟（P小于或等于0.05）也没有显著影响苯酚（I期和II期）的生物转化速率。在这些微粒体检测中，三卡因处理的虹鳟鱼与对照组之间的P450蛋白水平或7-乙氧基间苯二酚- o -去乙基酶（EROD）活性也没有显著的统计学差异（P小于或等于0.05）。Elsevier Science B.V.出版
The effect of tricaine methanesulfonate (MS222) on rainbow trout liver biotransformation rates was investigated with a microsomal model; an in vitro preparation that can be employed with or without the use of an anaesthetic. Two experimental sets of rainbow trout microsomes were tested; one representing in vivo or surgical tricaine exposures and the other representing in vitro tissue/organ collection tricaine exposures. Microsomal incubations were performed on these two experimental groups with phenol as substrate to assess the effects of tricaine on Phase I (ring-hydroxylation) and H (glucuronidation) liver biotransformation by monitoring production of hydroquinone (HQ) catechol (CAT), and phenylglucuronide (PG). The use of a 2-h 100 mg/l exposure of tricaine for surgical anesthesia with or without 24-h recovery did not significantly (P less than or equal to 0.05) affect rates of phenol (Phase I and II) biotransformation rates; nor, did the 5-min 300 mg/l tricaine exposure for isolated organ/tissue collection significantly (P less than or equal to 0.05) affect phenol (Phase I and II) biotransformation rates. There were also no significant statistical differences (P less than or equal to 0.05) in P450 protein levels, or 7-ethoxyresorufin-O-deethylase (EROD) activity in these microsomal assays between any of the tricaine treated rainbow trout and controls. Published by Elsevier Science B.V.