Analysis of HIV-1 load in blood, semen and saliva: Evidence for different viral compartments in a cross-sectional and longitudinal study
Analysis of HIV-1 load in blood, semen and saliva: Evidence for different viral compartments in a cross-sectional and longitudinal study
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DOI：
10.1097/00002030-199612000-00001
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发表时间：
1996-12-01
期刊：
影响因子：
3.8
通讯作者：
Piazza, M
中科院分区：
文献类型：
--
作者：
Liuzzi, G;Chirianni, A;Piazza, M
目的：为了量化的HIV-1载量（测量为病毒RNA的拷贝数/ml，使用竞争性逆转录-聚合酶链反应）在血液中，精液和唾液中，并寻找之间的关系的病毒负荷，临床和免疫状态和抗逆转录病毒therapy.Methods：外周血，精液和整个唾液样本收集从26个抗HIV-1血清阳性患者选择的横断面研究。结果：26例患者中有26例血浆、25例精液和24例唾液中检测到HIV-1 RNA。血浆中HIV-1拷贝数中位数为14817/ml（范围：167-254880），精液中为515/ml（范围：0-196050），唾液中为162/ml（范围：0-72080）。精液和唾液中病毒载量均显著低于血浆（P < 0.0001）。血浆和唾液中HIV-1 RNA水平相关（P < 0.05），精液中HIV-1 RNA水平与血浆和唾液均无相关性。有症状者血浆HIV-1拷贝数显著高于无症状者（P < 0.05）。CD 4+细胞计数< 200 x 10（6）/l的受试者血浆和唾液HIV-1 RNA水平高于CD 4+细胞计数> 200 x 10（6）/l的受试者（P < 0.05）。血浆，精液或唾液中的HIV-1 RNA载量与抗逆转录病毒therapy.Conclusions：血浆和精液载量之间的相关性的情况下，表明精液和血液是不同的病毒车厢。精液中的病毒载量与HIV感染的临床分期或CD 4+淋巴细胞计数无关。因此，HIV-1感染的受试者在免疫缺陷的所有阶段都具有潜在的传染性，必须始终采取适当的预防措施以防止HIV的性传播。
Objectives: To quantify the HIV-1 load (measured as copies of viral RNA/ml using competitive reverse transcription-polymerase chain reaction) in blood, semen and saliva and to look for relationships between the viral burden, the clinical and immunological status and antiretroviral therapy.Methods: Peripheral blood, semen and whole saliva samples were collected from 26 anti-HIV-1-seropositive patients selected for a cross-sectional study. Nine of the 26 patients provided samples of the three biological fluids for a longitudinal study.Results: HIV-1 RNA was detected in 26 out of 26 samples of plasma, in 25 out of 26 samples of semen and in 24 out of 25 samples of saliva. The median number of HIV-1 copies in plasma was 14817/ml (range: 167-254880), in semen was 515/ml (range: 0-196050) and in saliva was 162/ml (range: 0-72080). The viral load in semen and in saliva was significantly lower than in plasma (P < 0.0001). The HIV-1 RNA levels in plasma and in saliva were correlated (P < 0.05), but levels in semen were not correlated with either plasma or saliva levels. The HIV-1 copy number in plasma was significantly higher in symptomatic patients than in asymptomatic subjects (P < 0.05). Plasma and saliva HIV-1 RNA levels were higher in subjects with a CD4+ cell count < 200 x 10(6)/l than in subjects with a CD4+ cell count > 200 x 10(6)/l (P < 0.05). The HIV-1 RNA load in either plasma, semen or saliva is not related to antiretroviral therapy.Conclusions: The absence of a correlation between plasma and semen loads suggests that semen and blood are distinct viral compartments. Viral load in semen is not related to the clinical stage of HIV infection or to the CD4+ lymphocyte count. Consequently, HIV-1-infected subjects are potentially infectious at all stages of immunodeficiency and adequate precautions must always be taken to prevent the sexual transmission of HIV.