Influence of solvent viscosity on the rate of hydrolysis of dipeptides by carboxypeptidase Y
Influence of solvent viscosity on the rate of hydrolysis of dipeptides by carboxypeptidase Y
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DOI：
10.1002/poc.752
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发表时间：
2004-05-01
影响因子：
1.8
通讯作者：
Ohkata, K
中科院分区：
文献类型：
--
作者：
Kanosue, Y;Kojima, S;Ohkata, K
研究了溶剂粘度对羧肽酶Y水解一系列二肽（Z-Phe-Gly、Z-Phe-Sar、Z-Phe-Ala、Z-Phe-NMeAla、Z-Phe-Aib和Z-Phe-Pro）的影响。溶剂粘度对酶促水解的影响表明，而所有的k（猫）值随粘度下降，那些N-烷基肽比那些N-H肽下降。的动力学行为意味着参与的构象变化的酶的“诱导适合”的过程。版权所有（C）2004约翰威利父子有限公司。
The influence of solvent viscosity on the rate of enzymatic hydrolysis of a series of dipeptides (Z-Phe-Gly, Z-Phe-Sar, Z-Phe-Ala, Z-Phe-NMeAla, Z-Phe-Aib and Z-Phe-Pro) by carboxypeptidase Y was investigated. The effect of solvent viscosity on the enzymatic hydrolysis revealed that whereas all k(cat) values decreased with viscosity, those of the N-alkyl peptides decreased more than those of the N-H peptides. The kinetic behaviour implies the involvement of conformational changes of the enzyme in terms of the 'induced-fit' process. Copyright (C) 2004 John Wiley Sons, Ltd.