The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe.
The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe.
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发表时间：
1988-03
影响因子：
4
通讯作者：
I. Hagan;J. Hyams
中科院分区：
文献类型：
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作者：
I. Hagan;J. Hyams
我们用间接免疫荧光显微镜研究了分裂酵母裂糖酵母在细胞分裂周期中发生的微管组织变化。在间期，细胞质微管群独立于纺锤极体（SPB），形成在细胞尖端之间延伸的阵列。这些微管参与细胞核在细胞赤道的定位和细胞极性的建立。在有丝分裂时，间相阵列消失，被延伸到现在复制的spb之间的核内纺锤体所取代。纺锤体的伸长可以看到从spb细胞质面发出的星状微管的出现。这些持续到后期结束，此时纺锤体微管解聚，细胞赤道的两个微管组织中心（MTOCs）重新建立间相阵列。我们利用各种细胞分裂周期突变体的独特特性，进一步研究了这些不同微管阵列的功能及其时间和位置控制。
We have characterized the changes in microtubule organization that occur through the cell division cycle of the fission yeast Schizosaccharomyces pombe by indirect immunofluorescence microscopy. During interphase, groups of cytoplasmic microtubules, independent of the spindle pole body (SPB), form an array extending between the cell tips. These microtubules are involved in positioning the nucleus at the cell equator and in the establishment of cell polarity. At mitosis, the interphase array disappears and is replaced by an intranuclear spindle extending between the now duplicated SPBs. Elongation of the spindle sees the appearance of astral microtubules emanating from the cytoplasmic face of the SPBs. These persist until the end of anaphase whereupon the spindle microtubules depolymerize and two microtubule organizing centres (MTOCs) at the cell equator re-establish the interphase array. We have used the unique properties of various cell division cycle mutants to investigate further the function of these different microtubule arrays and their temporal and positional control.