The gene for the beta subunit of bovine luteinizing hormone encodes a gonadotropin mRNA with an unusually short 5'-untranslated region.
The gene for the beta subunit of bovine luteinizing hormone encodes a gonadotropin mRNA with an unusually short 5'-untranslated region.
复制标题
DOI：
10.1016/s0021-9258(18)88889-5
复制
发表时间：
1985-06
期刊：
影响因子：
--
通讯作者：
J. Virgin;B. Silver;A. Thomason;J. Nilson
中科院分区：
文献类型：
--
作者：
J. Virgin;B. Silver;A. Thomason;J. Nilson
编码牛黄体生成素(LH)β亚基的cDNA和基因组克隆已被分离和鉴定。测定了整个基因的核苷酸序列和776个碱基对的5‘侧翼序列。通过引物延伸和S1核酸酶保护分别定位了mRNA帽和多聚腺苷酸化位点。牛的黄体生成素β基因全长不到1.1kb，有三个外显子编码550个核苷酸的信使核糖核酸(不包括聚(A)尾)。牛的促黄体生成素β是单拷贝基因，而人的促黄体生成素β是促黄体生成素/绒毛膜促性腺激素β亚单位多基因家族的成员。将牛黄体生成素β基因与人黄体生成素β/绒毛膜促性腺激素基因家族进行比较，发现在基因内部和5‘-侧翼序列中都有高度的核苷酸序列同源性。尽管有这种广泛的序列保守，但这两个物种在启动子位点的选择上存在重大差异。结果，牛的黄体生成素β基因产生了一个通常只有6-11个核苷酸的短5‘非翻译区的信使核糖核酸。
Both cDNA and genomic clones encoding the beta subunit of bovine luteinizing hormone (LH) have been isolated and characterized. The nucleotide sequence was determined for the entire gene and 776 base pairs of 5'-flanking sequence. The mRNA cap site and polyadenylation site were mapped by primer extension and S1 nuclease protection, respectively. The bovine LH beta spans less than 1.1 kilobase pairs and has three exons encoding a 550 nucleotide mRNA (excluding the poly(A) tail). Bovine LH beta is a single-copy gene, in contrast to human LH beta, which is a member of the LH/chorionic gonadotropin beta subunit multigene family. Comparison of the bovine LH beta gene with the human LH beta/chorionic gonadotropin gene family reveals a high degree of nucleotide sequence homology, both within the genes and in the 5'-flanking sequences. Despite this extensive sequence conservation, there is a major difference between the two species in the selection of a promoter site. As a result, the bovine LH beta gene produces an mRNA with an usually short 5'-untranslated region of only 6-11 nucleotides.