Increased platelet oxidative metabolism, blood oxidative stress and neopterin levels after ultra-endurance exercise
Increased platelet oxidative metabolism, blood oxidative stress and neopterin levels after ultra-endurance exercise
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DOI：
10.1080/02640414.2013.797098
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发表时间：
2014-01-02
影响因子：
3.4
通讯作者：
Latini, Alexandra
中科院分区：
文献类型：
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作者：
De Lucas, Ricardo Dantas;Caputo, Fabrizio;Latini, Alexandra
本研究的目的是确定参加巴西超耐力多项运动比赛的运动员的肌肉损伤、炎症反应和氧化应激血液标记物。 11 名训练有素的男性运动员（34.3 +/- 3.1 岁，74.0 +/- 7.6 公斤；172.2 +/- 5.1 厘米）参加了这项研究并进行了比赛，比赛包括约 90 公里的交替越野跑、山地自行车和皮划艇。赛前 12 小时至赛后 15 分钟，抽取 10mL 血样以测量以下参数：乳酸脱氢酶和肌酸激酶活性、脂质过氧化、过氧化氢酶活性、蛋白质羰基化、呼吸链复合物 I、II 和 IV 活性、耗氧量和新蝶呤浓度。比赛结束后，血浆乳酸脱氢酶和肌酸激酶活性显着升高。赛后样本中红细胞 TBA-RS 水平和血浆蛋白羰基化显着增加。此外，赛后富含血小板的血浆中线粒体复合物 II 的活性和耗氧量也有所增加。这些生化参数的改变伴随着血浆新蝶呤水平的增加。超耐力事件引发全身炎症（新蝶呤增加），并伴有明显的氧化应激，可能是由于氧化代谢增加（氧化线粒体功能增加）所致。这在长时间运动期间可能是有利的，主要是为了在线粒体水平上有效地氧化底物，即使在诱导组织损伤时也是如此。
The purpose of the present investigation was to identify muscle damage, inflammatory response and oxidative stress blood markers in athletes undertaking the ultra-endurance MultiSport Brazil race. Eleven well-trained male athletes (34.3 +/- 3.1 years, 74.0 +/- 7.6kg; 172.2 +/- 5.1cm) participated in the study and performed the race, which consisted of about 90km of alternating off-road running, mountain biking and kayaking. Twelve hours before and up to 15 minutes after the race a 10mL blood sample was drawn in order to measure the following parameters: lactate dehydrogenase and creatine kinase activities, lipid peroxidation, catalase activity, protein carbonylation, respiratory chain complexes I, II and IV activities, oxygen consumption and neopterin concentrations. After the race, plasma lactate dehydrogenase and creatine kinase activities were significantly increased. Erythrocyte TBA-RS levels and plasma protein carbonylation were markedly augmented in post-race samples. Additionally, mitochondrial complex II activity and oxygen consumption in post-race platelet-rich plasma were also increased. These altered biochemical parameters were accompanied by increased plasma neopterin levels. The ultra-endurance event provoked systemic inflammation (increased neopterin) accompanied by marked oxidative stress, likely by increasing oxidative metabolism (increased oxidative mitochondrial function). This might be advantageous during prolonged exercise, mainly for efficient substrate oxidation at the mitochondrial level, even when tissue damage is induced.