Highly aligned skeletal muscle fibers
Highly aligned skeletal muscle fibers
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高度排列的骨骼肌纤维
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
S. Takeuchi
中科院分区：
文献类型：
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作者：
Y. Shimoyama;H. Onoe;Y. Tsuda;S. Takeuchi
我们提出高度排列的骨骼肌纤维（图1）。我们发现，纤维蛋白水凝胶纤维的纺织品是高度取向的纤维的纵向方向，通过使用微玻璃毛细管的微流体通道。我们还证实，接种的骨骼肌细胞C2C12迁移到纤维中，并与纤维方向对齐，而没有任何复杂的平版印刷图案。此外，纤维中的骨骼肌细胞分化为多核肌管。作为演示，我们通过将我们的束和拉伸的骨骼肌纤维整合到生物相容性聚合物手指来设计人工手指模型。我们相信，这将是制造由高度排列的骨骼肌细胞收缩驱动的软材料手指的重要步骤。
We propose highly aligned skeletal muscle fibers (Fig.1). We found that the textiles of a fibrin hydrogel fiber are highly orientated to the the longitudinal direction of the fiber by using a micro fluidic channel of a micro glass capillary tube. We also confirmed that the seeded skeletal muscle cells, C2C12, migrated into the fiber and aligned to the fiber direction without any complex lithographic patterning. Furthermore, the skeletal muscle cells in the fiber were differentiated to multinucleus myotubes. As a demonstration, we engineered artificial finger models by integrating our bundle and stretched skeletal muscle fibers to biocompatible polymer fingers. We believe that this would be an essential step to fabricate a soft material finger driven by the contraction of highly aligned skeletal muscle cells.