Demonstration of Ia antigens on mouse intestinal epithelial cells by immunoferritin labeling
Demonstration of Ia antigens on mouse intestinal epithelial cells by immunoferritin labeling
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通过免疫铁蛋白标记证明小鼠肠上皮细胞上的 Ia 抗原
DOI：
10.1007/bf01561459
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发表时间：
1979
期刊：
影响因子：
3.2
通讯作者：
I. McKenzie
中科院分区：
文献类型：
--
作者：
E. Parr;I. McKenzie
用只与H-2复合物I区抗原反应的抗血清进行免疫铁蛋白标记，研究了几种表达H-2抗原的上皮细胞。脾淋巴细胞用于测试标记系统和上皮细胞解离程序对Ia抗原的影响。免疫球蛋白阳性的B_（10.BR）淋巴细胞用抗LAK血清（A.TH抗A.TL血清被BALB/c和B_（10.D2）细胞吸收）标记，而同源的B_（10.D2）淋巴细胞未标记。标记的Ia抗原在活的B10.BR淋巴细胞上的分布是斑片状的，而在标记前用高碘酸盐-赖氨酸-多聚甲醛固定的细胞上，标记的分布是连续的。固定明显地将Ia抗原固定在淋巴细胞膜上。胰蛋白酶和胶原酶，用于上皮细胞的解离过程中，没有明显的影响淋巴细胞的Ia抗原。研究的上皮细胞包括小肠的柱状吸收细胞、子宫衬里上皮、气管刷状细胞、胰腺外分泌细胞和导管细胞。这些细胞在从其各自的组织解离之前被固定。Ia抗原仅在小肠柱状吸收细胞上检测到。这些细胞用仅与H-2复合物的K-末端反应的抗血清同样标记良好。在这两种情况下，同源对照肠细胞未标记。因此，肠上皮细胞似乎表达H-2复合物的Ia、K和可能的D区，而其他上皮细胞类型仅表达K和D抗原。在固定的肠上皮细胞上，Ia和H-2K抗原连续分布在包括粘附小带在内的侧细胞膜和基底细胞膜上，但在顶端微绒毛膜和闭合小带上不存在抗原。
Several kinds of epithelial cells that express H-2 antigens were studied by immunoferritin labeling with an antiserum reacting only with antigens of theI region of theH-2 complex. Spleen lymphocytes were used to test the labeling system and the effect of the epithelial cell dissociation procedure on Ia antigens. Immunoglobulin-positive B10.BR lymphocytes were labeled with an anti-lak serum (A.TH anti-A.TL serum absorbed with BALB/c and B10.D2 cells), while congenic B10.D2 lymphocytes were unlabeled. The distribution of labeled Ia antigens on living B10.BR lymphocytes was patchy, while on cells fixed in periodate-lysine-paraformaldehyde before labeling, the distribution of label was continuous. Fixation evidently immobilized Ia antigens in the lymphocyte membrane. Trypsin and collagenase, as used in the epithelial cell dissociation procedure, had no discernible effect on the Ia antigens of lymphocytes. The epithelial cells studied included the columnar absorptive cells of the small intestine, uterine lining epithelium, tracheal brush cells, and pancreatic exocrine and duct cells. These cells were fixed before dissociation from their respective tissues. Ia antigens were detected only on the columnar absorptive cells of the small intestine. These cells labeled equally well with an antiserum reacting only with theK -end of theH-2 complex. In both cases, congenic control intestinal cells were unlabeled. Thus, intestinal epithelial cells appear to express theIa, K, and presumablyD regions of theH-2 complex, while the other epithelial cell types express only the K and D antigens. On fixed intestinal epithelial cells, Ia and H-2K antigens were continuously distributed on the lateral and basal cell membranes including the zonula adherens, but the antigens were absent from the apical microvillous membrane and the zonula occludens.