COPPER DEFICIENCY ALTERS ISOMYOSIN TYPES AND LEVELS OF LAMININ, FIBRONECTIN AND CYTOCHROME-C-OXIDASE SUBUNITS FROM RAT HEARTS
COPPER DEFICIENCY ALTERS ISOMYOSIN TYPES AND LEVELS OF LAMININ, FIBRONECTIN AND CYTOCHROME-C-OXIDASE SUBUNITS FROM RAT HEARTS
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DOI：
10.1016/0305-0491(94)00226-k
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发表时间：
1995-05-01
影响因子：
2.2
通讯作者：
MEDEIROS, DM
中科院分区：
文献类型：
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作者：
LIAO, ZM;ALLRED, J;MEDEIROS, DM
通过凝胶电泳和蛋白质印迹法研究断奶后分别喂养各自饮食 3 周的对照和缺铜 Sprague-Dawley 大鼠中的心蛋白（如层粘连蛋白、纤连蛋白、细胞色素 C 氧化酶和异肌球蛋白类型）的相对含量，异肌球蛋白类型似乎从 V-1 转变为男女缺铜大鼠中更高水平的 V-3。雄性缺铜大鼠心脏纤连蛋白水平升高，层粘连蛋白水平降低，心脏肥大和贫血。与各自的对照组相比，喂食缺铜饮食的雄性和雌性大鼠的细胞色素c氧化酶（CCO）IV亚基水平较低，肝铜水平较低，心脏重量比较高。
The relative amounts of caridac proteins such as laminin, fibronectin, cytochrome c oxidase, and isomyosin types were studied by gel electrophoresis and Western blotting in control and copper-deficient Sprague-Dawley rats of both sexes fed their respective diets from weanling for 3 weeks, Isomyosin types appeared to shift from V-1, to greater levels of V-3 in copper deficient rats for both genders. Male copper deficient rats had increased cardiac levels of fibronectin, decreased laminin levels, cardiac hypertrophy and anemia. Both male and female rats fed copper-deficient diet had lower levels of cytochrome c oxidase (CCO) subunit IV, and low liver copper, and high heart-to-body weight ratios compared with their respective controls.