MicroRNA-7 functions as a tumor-suppressor gene by regulating ILF2 in pancreatic carcinoma.
MicroRNA-7 functions as a tumor-suppressor gene by regulating ILF2 in pancreatic carcinoma.
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DOI：
10.3892/ijmm.2017.2894
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发表时间：
2017-04
影响因子：
5.4
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Bi Y;Shen W;Min M;Liu Y
白细胞介素增强子结合因子2（IL F2）在胰腺癌中表达显著上调，并参与胰腺癌的发病机制。因此，ILF 2可能是治疗的潜在靶点。然而，ILF 2在胰腺癌中的调节机制仍然很难理解。在本研究中，我们证明了ILF 2作为癌基因在胰腺癌PANC-1细胞中起作用，并调节上皮间质转化（EMT）相关基因。microRNA-7（miR-7）抑制PANC-1细胞ILF 2 mRNA表达和蛋白水平。与ILF 2相反，miRNA-7在PANC-1细胞中起肿瘤抑制基因的作用，并负调控EMT相关基因。姜黄素是一种从姜黄根茎中提取的多酚类天然产物，是一种很有前途的抗癌药物。我们发现姜黄素处理增加了PANC-1细胞中miR-7的表达并抑制了ILF 2蛋白。因此，我们确定了ILF 2作为姜黄素的一个新的下游靶基因。结果表明，ILF 2受miR-7的调控，提示miR-7的下调可能是胰腺癌中ILF 2过表达的重要因素。
Interleukin enhancer binding factor 2 (ILF2) has been found to be markedly upregulated in pancreatic carcinoma and is involved in the pathogenesis of pancreatic carcinoma. Thus, ILF2 may be a potential target for therapy. Yet, the regulatory mechanisms of ILF2 in pancreatic carcinoma remain largely elusive. In the present study, we demonstrated that ILF2 functioned as an oncogene and regulated epithelial-mesenchymal transition (EMT)-associated genes in pancreatic carcinoma PANC-1 cells. MicroRNA-7 (miR-7) suppressed ILF2 mRNA expression and the protein level in PANC-1 cells. Contrary to ILF2, miRNA-7 functioned as a tumor-suppressor gene and negatively regulated EMT-associated genes in the PANC-1 cells. Curcumin, a polyphenol natural product isolated from the rhizome of the plant Curcuma longa, has emerged as a promising anticancer therapeutic agent. We found that treatment with curcumin increased miR-7 expression and suppressed ILF2 protein in the PANC-1 cells. Thus, we identified ILF2 as a new downstream target gene of curcumin. The results revealed that ILF2 is regulated by miR-7 and suggest that downregulation of miR-7 may be an important factor for the ILF2 overexpression in pancreatic carcinoma.