LIVER PLASMA-MEMBRANES AND PROTEOGLYCAN PREPARED THEREFROM INHIBIT THE GROWTH OF HEPATOMA-CELLS INVITRO
LIVER PLASMA-MEMBRANES AND PROTEOGLYCAN PREPARED THEREFROM INHIBIT THE GROWTH OF HEPATOMA-CELLS INVITRO
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DOI：
10.1016/0005-2736(81)90283-2
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
TERAYAMA, H
中科院分区：
文献类型：
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作者：
KAWAKAMI, H;TERAYAMA, H
大鼠肝脏或肾脏的质膜在体外抑制肝癌 (AH-130) 细胞的生长。 AH-130质膜或红细胞血影对AH-130细胞生长的抑制效果较差。热处理或温和蛋白酶（木瓜蛋白酶或菠萝蛋白酶，但不是胰蛋白酶或链霉蛋白酶）处理会丧失肝脏质膜的抑制活性；唾液酸酶处理或乙醇/乙醚脱脂后保留。从肝质膜制备的蛋白多糖（硫酸蛋白乙酰肝素）抑制AH-130细胞的生长，但硫酸乙酰肝素活性较低。肝脏质膜的抑制活性似乎不仅仅归因于与质膜相关的硫酸蛋白乙酰肝素。初步研究表明，分子量为40,000的成分可能是肝质膜的主要抑制成分。
Plasma membranes from rat liver or kidney inhibited the growth of hepatoma (AH-130) cells in vitro. AH-130 plasma membranes or erythrocyte ghosts inhibited the growth of AH-130 cells less effectively. The inhibitory activity of liver plasma membranes was lost by heat treatment or mild protease (papain or bromelin, but not trypsin or pronase) treatment; it was retained after sialidase treatment or delipidation by ethanol/ether. Proteoglycan (proteoheparan sulfate) prepared from liver plasma membranes inhibited the growth of AH-130 cells, but heparan sulfate was less active. The inhibitory activity of liver plasma membranes seemed not to be ascribable solely to proteoheparan sulfate associated with plasma membranes. Preliminary investigations suggested that the MW 40,000 component may be a major inhibitory principle in liver plasma membranes.