Tongue coating microbiome data distinguish patients with pancreatic head cancer from healthy controls
Tongue coating microbiome data distinguish patients with pancreatic head cancer from healthy controls
复制标题
舌苔微生物组数据将胰头癌患者与健康对照区分开来。
DOI：
10.1080/20002297.2018.1563409
复制
发表时间：
2019-01-01
影响因子：
4.5
通讯作者：
Li, Lanjuan
中科院分区：
文献类型：
--
作者：
Lu, Haifeng;Ren, Zhigang;Li, Lanjuan
摘要背景：微生物群在人类致癌过程中起着至关重要的作用。胰头癌（PHC）相关的舌苔微生物群失调尚未明确定义。目的：我们的目的是揭示PHC患者舌苔微生物群中细菌组成的变化。设计：使用16S rRNA基因测序技术分析30名PHC患者和25名健康对照的舌苔微生物群。结果：PHC患者舌苔的微生物组多样性显著增加，如Shannon，Simpson，逆Simpson，Obs和基于发病率的覆盖率估计所示。主成分分析显示，PHC患者与健康对照组和肝癌患者相比，舌苔微生物群明显不同。线性判别分析效应大小显示，在PHC患者的舌苔中，Leptotrichia、Fusobacterium、Rothia、Actinomyces、Corynebacterium、Atopobium、Peptostreatum、Catonella、Oribacterium、Filifactor、Campylobacter、Moraxella和Tannerella的比例较高，而在健康对照的舌苔微生物群中，嗜血杆菌、卟啉单胞菌和副Revovetella的比例较高。其中，嗜血杆菌属、卟啉单胞菌属、纤毛菌属和梭杆菌属可将PHC患者与健康人区分开来，链球菌属和SR1可将PHC患者与肝癌患者区分开来。结论：这些发现确定了PHC患者舌苔的微生物群失调，并提供了对人类微生物组与胰腺癌之间关系的深入了解。
ABSTRACT Background: The microbiota plays a critical role in the process of human carcinogenesis. Pancreatic head carcinoma (PHC)-associated tongue coating microbiome dysbiosis has not yet been clearly defined.Objective: Our aim is to reveal the bacterial composition shifts in the microbiota of the tongue coat of PHC patients.Design: The tongue coating microbiota was analyzed in 30 PHC patients and 25 healthy controls using 16S rRNA gene sequencing technology.Results: The microbiome diversity of the tongue coat in PHC patients was significantly increased, as shown by the Shannon, Simpson, inverse Simpson, Obs and incidence-based coverage estimators. Principal component analysis revealed that PHC patients were colonized by remarkably different tongue coating microbiota than healthy controls and liver cancer patients. Linear discriminant analysis effect size revealed that Leptotrichia, Fusobacterium,Rothia, Actinomyces, Corynebacterium, Atopobium, Peptostreptococcus, Catonella, Oribacterium, Filifactor, Campylobacter, Moraxella and Tannerella were overrepresented in the tongue coating of PHC patients, and Haemophilus, Porphyromonas and Paraprevotella were enriched in the tongue coating microbiota of healthy controls. Strikingly, Haemophilus, Porphyromonas, Leptotrichia and Fusobacterium could distinguish PHC patients from healthy subjects, and Streptococcus and SR1 could distinguish PHC patients from liver cancer patients. Conclusions: These findings identified the microbiota dysbiosis of the tongue coat in PHC patients, and provide insight into the association between the human microbiome and pancreatic cancer.