The Study of Myogenin Expression in Denervated Human Skeletal Muscles
The Study of Myogenin Expression in Denervated Human Skeletal Muscles
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去神经人类骨骼肌中肌生成素表达的研究
DOI：
10.1177/147323001103900205
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发表时间：
2011-03-01
影响因子：
1.6
通讯作者：
Xu, J. G.
中科院分区：
文献类型：
--
作者：
Chen, L.;Huang, H. W.;Xu, J. G.
骨骼肌失神经支配后，由于神经传导中断和营养因子缺乏，最终导致萎缩。肌细胞生成素是一种成肌调节因子，在成肌细胞分化中起关键作用。已经证实了在去神经大鼠骨骼肌中肌细胞生成素表达的变化，但是在去神经人骨骼肌中肌细胞生成素的表达还没有报道。在去神经支配后的不同时间点分析人类肌肉样品，以评估肌细胞生成素表达的变化及其与骨骼肌萎缩的关系。失神经支配后，myogenin mRNA水平在7个月时达到峰值，是正常水平的37.5倍。表达水平然后下降到21和11倍的正常水平，分别在12和26个月后去神经支配。长时间的失神经支配导致病理变化，其特征在于完整的肌纤维数量减少。
Skeletal muscle denervation eventually causes atrophy as a result of interrupted nerve conduction and the lack of nutritional factors. Myogenin is a myogenic regulatory factor that plays a key role in myoblast differentiation. Changes in myogenin expression in denervated rat skeletal muscle have been demonstrated, but myogenin expression in denervated human skeletal muscle has not been reported. Human muscle samples were analysed at different time-points post-denervation to evaluate changes in myogenin expression and their relationship with skeletal muscle atrophy. Post-denervation, myogenin mRNA levels peaked at 7 months and were 37.5 times the normal level. Expression levels then declined to 21 and 11 times the normal level at 12 and 26 months post-denervation, respectively. Prolonged denervation resulted in pathological changes characterized by decreased numbers of intact muscle fibres.