Optimization boat-fruited of extraction process of crude polysaccharides from sterculia seeds by response surface methodology
Optimization boat-fruited of extraction process of crude polysaccharides from sterculia seeds by response surface methodology
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DOI：
10.1016/j.foodchem.2007.03.066
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发表时间：
2007-01-01
期刊：
影响因子：
8.8
通讯作者：
Gu, Xiaohong
中科院分区：
文献类型：
--
作者：
Wu, Yan;Cui, Steve W.;Gu, Xiaohong
采用响应面法（RSM）对茯苓粗多糖的提取工艺进行优化。采用中心组合设计法进行实验设计和结果分析，确定最佳提取条件。结果表明，提取温度、pH、提取时间和水籽比对粗多糖得率和纯度有显著影响，而除水籽比外的其他3个因素对粗多糖的相对粘度也有显著影响。根据RSM分析，最佳条件为：温度60 ~ 65℃，时间2.3 ~ 3.1 h, pH 7.0，水种比75:1。在优化条件下，实验值与模型预测值吻合较好。在最佳条件下制备的粗多糖碳水化合物（含糖醛酸）含量为58.2%，蛋白质含量为20%，水分含量为9%，灰分含量为4.5%。粗多糖由葡萄糖（22.6%）、鼠李糖（10.0%）、阿拉伯糖（7.9%）、半乳糖（5.0%）、木糖（0.8%）和半乳糖醛酸（11.8%）组成。(c) 2007年Elsevier Ltd.出版
Response surface methodology (RSM) was applied to optimize the extraction of crude polysaccharides from boat-fruited sterculia seeds. A central composite design was used for experimental design and analysis of the results to obtain the optimal extraction conditions. Extraction temperature, pH, extraction time and water to seed ratio were found to have a significant influence on the yield and purity of the extracted crude polysaccharides, while the three other factors except the water to seed ratio also significantly affected the relative viscosity. Based on the RSM analysis, optimum conditions were: temperature 60-65 degrees C, time 2.3-3.1 h, pH at 7.0 and water to seed ratio at 75:1. Under the optimized conditions, the experimental values were in close agreement with values predicted by the model. The crude polysaccharides prepared under optimum conditions contained 58.2% total carbohydrates (including uronic acids), 20% proteins, 9% moisture and 4.5% ash. The crude polysaccharides consisted of glucose (22.6%), rhamnose (10.0%), arabinose (7.9%), galactose (5.0%), xylose (0.8%) and galacturonic acid (11.8%). (c) 2007 Published by Elsevier Ltd.