Antimicrobial activity and protective properties of vaginal lactobacilli from healthy Bulgarian women
Antimicrobial activity and protective properties of vaginal lactobacilli from healthy Bulgarian women
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DOI：
10.1016/j.anaerobe.2007.08.003
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发表时间：
2007-10-01
期刊：
影响因子：
2.3
通讯作者：
Bakalov, Boris V.
中科院分区：
文献类型：
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作者：
Dimitonova, Silviya P.;Danova, Svetla T.;Bakalov, Boris V.
阴道乳杆菌作为一种有效的抵抗病原体入侵的屏障的作用引起了相当大的兴趣。本研究的目的是在体外评估20种最近鉴定的阴道乳酸杆菌保护阴道的能力。为了评估它们的重要性。抗菌素。血凝（HA）和聚集（Agg）活性，以及酸和过氧化氢（H2O2）的产生。被估计。8株菌株的无细胞培养在消除乳酸和H2O2的影响后显示出稳定的抗菌活性。三个分离的阴道乳酸菌表达了广谱的抗菌活性，包括革兰氏阴性病原体。菌株具有抗加德纳氏菌和2型单纯疱疹病毒的活性。所有分离株均产生H2O2。主动酸化培养基至pH 3.92 +/- 0.04。它被认为可以中和性传播感染病原体。大部分（75%）表达HA活性和不同的Agg表型，被认为是与入侵病原体竞争和宿主防御的重要特性。这些结果令人鼓舞，并促使进一步研究具有特征的活性菌株及其在预防阴道疾病方面的可能应用。(c) 2007 Elsevier Ltd.版权所有。
The role of vaginal Lactobacillas as an efficient barrier against invading pathogens is of considerable interest. The purpose of the present study was to assess in vitro the ability of 20 recently identified vaginal lactobacilli to protect the vagina. In order to evaluate their significance. the antimicrobial. hemagglutination (HA) and aggregation (Agg) activities, as well as acid and hydrogen peroxide (H2O2) production. were estimated. The cell-free Cultures of eight strains showed a stable antimicrobial activity after elimination of the putative effects of lactic acid and H2O2. Three of the isolated vaginal lactobacilli expressed a broad spectrum of anti-bacterial activity including Gram-negative pathogens. strains with anti-Gardnerella and anti-herpes simplex virus type 2 activities were found. All tested isolates were H2O2 producers. actively acidifying the growth media to pH 3.92 +/- 0.04. which is presumed to neutralize sexually transmitted infection pathogens. The major part (75%) expressed an HA activity and different Agg phenotypes, estimated as important properties in the competition with invading pathogens and in host defense. These results are encouraging and prompt further research of the characterized active strains and their possible application in prophylaxis of vaginal disorders. (c) 2007 Elsevier Ltd. All rights reserved.