Expression of Myh9 in the mammalian cochlea: localization within the stereocilia.
Expression of Myh9 in the mammalian cochlea: localization within the stereocilia.
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Myh9 在哺乳动物耳蜗中的表达：在静纤毛内的定位。
DOI：
10.1002/jnr.20993
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Lalwani,AnilK
中科院分区：
文献类型：
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作者：
Mhatre,AnandN;Li,Yan;Atkin,Graham;Maghnouj,Abdel;Lalwani,AnilK
非肌肉肌球蛋白IIA型或MYH9的突变与综合征性或非综合征性听力损失有关。MYH9在听觉器官中的生物学功能及其功能障碍的病理生理学仍有待确定。小鼠是研究MYH9在受其功能障碍影响的细胞和组织中的生物学作用的极好模型。理解MYH9在听力及其功能障碍中的作用的主要步骤是记录其在耳蜗（听觉器官）内的细胞和亚细胞定位。我们使用多克隆抗Myh9抗体描述了Myh9在小鼠耳蜗内的定位，该抗体针对与小鼠Myh9的C末端序列对应的18个氨基酸长的肽产生。抗Myh9抗体在多种不同小鼠组织匀浆的免疫印迹分析中鉴定出单一的220 kDa特异性免疫反应性条带。Myh9抗体与大鼠交叉反应，但不与人直系同源物交叉反应。Myh9主要在螺旋韧带以及Corti器官的感觉毛细胞中表达。耳蜗表面制备物的共聚焦显微镜，鉴定了内毛细胞和外毛细胞及其静纤毛内的Myh9。Myh9在静纤毛内的定位提高了MYH9突变可能通过破坏静纤毛结构而影响听力损失的可能性。© 2006 Wiley利斯公司
Mutations of non‐muscle myosin Type IIA or MYH9 are linked to syndromic or nonsyndromic hearing loss. The biologic function of MYH9 in the auditory organ and the pathophysiology of its dysfunction remain to be determined. The mouse represents an excellent model for investigating the biologic role of MYH9 in the cells and tissues affected by its dysfunction. A primary step toward the understanding of the role of MYH9 in hearing and its dysfunction is the documentation of its cellular and sub‐cellular localization within the cochlea, the auditory organ. We describe the localization of Myh9 within the mouse cochlea using a polyclonal anti‐Myh9‐antibody, generated against an 18 amino acid long peptide corresponding to the sequence at the C‐terminus of mouse Myh9. The anti‐Myh9 antibody identified a single, specific, immunoreactive band of 220 kDa in immunoblot analysis of homogenate from a variety of different mouse tissues. The Myh9 antibody cross‐reacts with the rat but not the human orthologue. Myh9 is expressed predominantly within the spiral ligament as well as in the sensory hair cells of the organ of Corti. Confocal microscopy of cochlear surface preparations, identified Myh9 within the inner and outer hair cells and their stereocilia. Localization of Myh9 within the stereocilia raises the possibility that mutations of MYH9 may effect hearing loss though disruption of the stereocilia structure. © 2006 Wiley‐Liss, Inc.