Nucleosome core histone complex isolated gently and rapidly in 2 M NaCl is octameric.
Nucleosome core histone complex isolated gently and rapidly in 2 M NaCl is octameric.
复制标题
在 2 M NaCl 中温和快速分离的核小体核心组蛋白复合物是八聚体。
DOI：
10.1073/pnas.76.10.5178
复制
发表时间：
1979
影响因子：
11.1
通讯作者：
H. Chandra
中科院分区：
文献类型：
--
作者：
M. Philip;M. Jamaluddin;R. Sastry;H. Chandra
A relatively stable specific complex of the chromatin core histones H2A, H2B, H3, and H4 has been obtained in 2 M NaCl/25 mM sodium phosphate buffer, pH 7.0. The histone core complex has an apparent specific volume of 0.73 ml/g. Its sedimentation coefficient was dependent on rotor speed (angular velocity, omega) and attained different stable values at low and high rotor speeds. The drop in sedimentation coefficient occurred sharply between omega 2 values of about 9 x 10(6) and 1.1 x 10(7) (radians/sec)2. The s020,w corresponding to zero angular velocity (1 atmosphere pressure) was 6.6 S +/- (SEM) 0.1 S. At high rotor speeds the value decreased to 3.8 S +/- 0.1 S. The core complex has a diffusion coefficient, D20,w, of 5.4 x 10(-7) cm2/sec and a molecular weight of 108,000 +/- (SD) 2500.
