Vascular endothelial growth factor-A and changes in a tumor-bearing mouse model with Lewis lung cancer
Vascular endothelial growth factor-A and changes in a tumor-bearing mouse model with Lewis lung cancer
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DOI：
10.3892/ol.2011.380
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发表时间：
2011-11-01
期刊：
影响因子：
2.9
通讯作者：
Wu, Ying-Tai
中科院分区：
文献类型：
--
作者：
Tsai, Meng-Shu;Chang, Cheng-Chi;Wu, Ying-Tai
血管内皮生长因子-A（VEGF-A）通过刺激血管生成影响肿瘤的生长和转移。本研究的目的是描述小鼠刘易斯肺癌（LLC）的特征，并与正常对照小鼠的血清VEGF-A水平进行比较。比较两组小鼠：一组皮下注射LLC细胞（n=16），另一组作为正常对照（n=6）。在接种前和接种后7、21和35天通过ELISA测量血清VEGF-A水平。在第35天评价肿瘤重量和转移状况。使用广义估计方程比较各组之间在一段时间内体重和血清VEGF-A浓度的变化。采用斯皮尔曼秩相关检验分析原发肿瘤与转移情况之间的关系。肿瘤接种后第35天的存活率为56.3%。接种后第35天，两组腓肠肌重量无差异[0.1315 +/- 0.0066 g vs. 0.1308 +/- 0.0069 g（正常对照）]。在荷瘤小鼠中，处死时体重增加较少（0.24 +/- 0.45 vs. 1.93 +/- 0.47 g，P=0.01），最终平均肿瘤体积和重量分别为4264.69 +/- 1038.32 mm（3）和3.70 +/- 0.83 g，肺和肝中的结节数分别为6.33（范围0-20）和2.22（范围0-11），血清VEGF-A水平显著高于对照小鼠。总之，较低的体重增加，肝和肺转移，以及VEGF-A水平升高是小鼠LLC的特征。
Vascular endothelial growth factor-A (VEGF-A) affects tumor growth and metastasis through stimulation of angiogenesis. The purpose of this study was to describe features of Lewis lung cancer (LLC) in mice and compare the serum VEGF-A levels with those of normal control mice. Two groups of mice were compared: one was subcutaneously injected with LLC cells (n=16) and the other served as the normal control (n=6). The serum VEGF-A levels were measured by ELISA prior to inoculation, and at 7, 21 and 35 days post-inoculation. The tumor weight and the metastatic condition were evaluated on day 35. Changes in body weight and serum VEGF-A concentration over a period of time were compared between the groups using generalized estimating equations. The relationship between the primary tumor and the metastatic condition was analyzed using the Spearman's rank correlation test. The survival rate was 56.3% on day 35 post-tumor inoculation. No difference was found between the groups with regard to gastrocnemius muscle weight on day 35 post-inoculation [0.1315 +/- 0.0066 g vs. 0.1308 +/- 0.0069 g (normal control)]. In tumor-bearing mice, the weight gain at sacrifice was less (0.24 +/- 0.45 vs. 1.93 +/- 0.47 g, P=0.01), the final mean tumor volume and weight were 4264.69 +/- 1038.32 mm(3) and 3.70 +/- 0.83 g, the number of nodules in the lungs and livers was 6.33 (range 0-20) and 2.22 (range 0-11), respectively, and the serum VEGF-A levels were significantly higher than those of control mice. In conclusion, lower body weight gain, metastasis in the liver and lungs, and elevated VEGF-A levels are features of LLC in mice.