INVIVO AND INVITRO ANALYSIS OF IMMUNITY TO TRICHINELLA-SPIRALIS IN MICE AND RATS
INVIVO AND INVITRO ANALYSIS OF IMMUNITY TO TRICHINELLA-SPIRALIS IN MICE AND RATS
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DOI：
10.1017/s0031182000047570
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发表时间：
1977-01-01
期刊：
影响因子：
2.4
通讯作者：
BLAIR, LS
中科院分区：
文献类型：
--
作者：
DESPOMMIER, DD;CAMPBELL, WC;BLAIR, LS
用T.使用来自成熟肌幼虫的无细胞匀浆的抗原级分，在大鼠中，经口攻击30天后，用250 μ g抗原免疫的动物体内的肌肉幼虫（135，000个）明显少于对照组（231，000个）。此外，来自免疫大鼠的7日龄蠕虫在体外24小时内比来自非免疫动物的蠕虫少48%（P < 0.001）的新生幼虫脱落。给小鼠注射10或100 μ g抗原。与未免疫的对照相比，用100 μ g抗原免疫的小鼠在经口攻击感染后第7天和第9天具有显著更少的蠕虫，而在攻击后第6-10天从免疫小鼠中回收的雌蠕虫在体外脱落更少的新生幼虫。用100 μ g抗原免疫的小鼠在攻击后30天比对照组（47，750）具有显著更少的肌幼虫（10，391）。用10 μ g抗原免疫小鼠在攻击后第7天或第9天未诱导蠕虫的统计学显著减少（P < 0.5）。来自接受10 μ g抗原的小鼠的成年雌性蠕虫仍然比来自对照小鼠的成虫脱落更少的幼虫（P < 0.05）。在攻击后30天，用10 μ g抗原免疫的小鼠比对照（39，000）具有显著更少的（13，700）可恢复肌幼虫。
Wistar-Furth strain male rats and CFW strain male mice were immunized against T. spiralis using an antigenic fraction derived from a cell-free homogenate of mature muscle larvae. In rats, animals immunized with 250 .mu.g of antigen harbored significantly fewer (135,000) muscle larvae 30 days after oral challenge than controls (231,000). Further 7 day old adult worms derived from immunized rats shed 48% fewer (P < 0.001) newborn larvae over a 24 h period in vitro than adult worms from non-immunized animals. Mice were injected with either 10 or 100 .mu.g of antigen. In comparison with non-immunized controls, mice immunized with 100 .mu.g of antigen harbored significantly fewer adult worms at days 7 and 9 after oral challenge infection, while female worms recovered from immune mice on days 6-10 after challenge shed fewer newborn larvae in vitro. Mice immunized with 100 .mu.g of antigen harbored significantly fewer (10,391) muscle larvae at 30 days after challenge than did controls (47,750). Immunization of mice with 10 .mu.g of antigen did not induce a statistically significant reduction in adult worms at either day 7 or 9 after challenge (P < 0.5). Adult female worms from mice receiving 10 .mu.g of antigen still shed fewer larvae than did adults from control mice (P < 0.05). Mice immunized with 10 .mu.g of antigen harbored significantly fewer (13,700) recoverable muscle larvae than did controls at 30 days after challenge (39,000).