Functional network analysis of the transcriptomes of mesenchymal stem cells derived from amniotic fluid, amniotic membrane, cord blood, and bone marrow
Functional network analysis of the transcriptomes of mesenchymal stem cells derived from amniotic fluid, amniotic membrane, cord blood, and bone marrow
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DOI：
10.1634/stemcells.2007-0023
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发表时间：
2007-01-01
期刊：
影响因子：
5.2
通讯作者：
Soong, Yung-Kuei
中科院分区：
文献类型：
--
作者：
Tsai, Ming-Song;Hwang, Shiaw-Min;Soong, Yung-Kuei
使用高密度寡核苷酸微阵列和功能网络分析，我们研究了来自四个不同来源的MSC是否单独表现出独特的基因表达谱，然后将所有MSC的基因表达谱与胎儿器官的基因表达谱进行比较。我们的研究结果表明，在同一来源的各组间充质干细胞中，基因表达水平的变异性小于不同来源组之间的变异性。功能基因组学研究揭示了不同来源的MSC的特定作用。我们的研究结果表明，羊水间充质干细胞可能通过上调催产素和凝血酶受体启动与子宫的相互作用。羊膜间充质干细胞可能通过调节内皮素、脑啡肽酶、缓激肽受体和心房利钠肽的网络来维持体液和电解质的稳态。脐血间充质干细胞可能参与先天免疫系统作为新生儿防御系统对最早遇到的病原体。成人骨髓间充质干细胞可能是一个重要的来源，不仅是所有的血液谱系，但也骨形成。然而，尽管在不同来源的MSC中观察到不同的基因表达谱，但在这四种MSC中保留了一组核心基因表达谱。与胎儿器官相比，所有MSC的核心签名转录组包括参与细胞外基质和粘附调节、转化生长因子β受体信号传导和Wnt信号传导途径的基因。
Using high-density oligonucleotide microarrays and functional network analyses, we examined whether MSCs derived from four different origins exhibited unique gene expression profiles individually and then compared the gene expression profiles of all MSCs with those of fetal organs. Our results indicated that within each group of MSCs from the same origin, the variability of the gene expression levels was smaller than that between groups of different origins. Functional genomic studies revealed the specific roles of MSCs from different origins. Our results suggest that amniotic fluid MSCs may initiate interactions with the uterus by upregulating oxytocin and thrombin receptors. Amniotic membrane MSCs may play a role in maintaining homeostasis of fluid and electrolytes by regulating the networks of endothelin, neprilysin, bradykinin receptors, and atrial natriuretic peptide. Cord blood MSCs may be involved in innate immune systems as the neonatal defense system against the earliest encountered pathogens. Adult bone marrow MSCs may be an important source not only of all blood lineages but also of bone formation. However, in spite of the different gene expression profiles seen in MSCs derived from different origins, a set of core gene expression profiles was preserved in these four kinds of MSCs. The core signature transcriptomes of all MSCs, when contrasted against those of fetal organs, included genes involved in the regulation of extracellular matrix and adhesion, transforming growth factor-beta receptor signaling, and the Wnt signaling pathways.