Early effects of salt stress on the physiological and oxidative status of Cakile maritima (halophyte) and Arabidopsis thaliana (glycophyte)
Early effects of salt stress on the physiological and oxidative status of Cakile maritima (halophyte) and Arabidopsis thaliana (glycophyte)
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DOI：
10.1111/j.1399-3054.2011.01450.x
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发表时间：
2011-06-01
影响因子：
6.4
通讯作者：
Abdelly, Chedly
中科院分区：
文献类型：
--
作者：
Ellouzi, Hasna;Ben Hamed, Karim;Abdelly, Chedly
对两种耐盐能力不同的十字花科植物Cakile maritima(盐生植物)和拟南芥(含糖植物)在盐胁迫下诱导的生理和氧化状态的早期变化进行了监测。比较了400 mM和100 mM盐胁迫下海藻的生长反应和抗氧化防御能力。盐度引起的早期生长减慢，在毛虾中没有那么明显，在海藻中。两种植物叶片中过氧化氢(H_2O_2)的最大值均出现在盐处理开始后4小时。在此之后，海桑的过氧化氢浓度迅速下降，而海桑的过氧化氢浓度保持在较高水平。相应地，超氧化物歧化酶、过氧化氢酶和过氧化物酶活性在处理4h后升高，此后下降。然而，无论处理的时间长短，处理组的这些酶的活性仍然高于对照。两种植物的丙二醛(MDA)浓度在盐胁迫24 h时达到最大值。同样，maritima中的丙二醛水平后来下降，但在A.thaliana中保持较高水平。α-生育酚的含量在盐胁迫期间保持不变，在盐胁迫的前24小时内下降，在盐胁迫后一直维持在较低的水平。结果清楚地表明，与海桑相比，海桑可以迅速进化出生理和抗氧化机制，以适应盐和管理氧化应激。这可能至少部分解释了盐生植物和糖生植物在耐盐性上的差异。
Early changes in physiological and oxidative status induced by salt stress were monitored in two Brassicaceae plants differing in their tolerance to salinity, Cakile maritima (halophyte) and Arabidopsis thaliana (glycophyte). Growth response and antioxidant defense of C. maritima under 400 mM NaCl were compared with those of A. thaliana exposed to 100 mM NaCl. Salinity induced early growth reduction that is less pronounced in C. maritima than in A. thaliana. Maximum hydrogen peroxide (H2O2) level occurred in the leaves of both species 4 h after the onset of salt treatment. A rapid decline in H2O2 concentration was observed thereafter in C. maritima, whereas it remained high in A. thaliana. Correlatively, superoxide dismutase, catalase and peroxidase activities increased at 4 h of treatment in C. maritima and decreased thereafter. However, the activity of these enzymes remained higher in treated plants than that in controls, regardless of the duration of treatment, in A. thaliana. The concentrations of malondialdehyde (MDA) reached maximum values at 24 h of salt stress in both species. Again, MDA levels decreased later in C. maritima, but remained high in A. thaliana. The contents of alpha-tocopherol remained constant during salt stress in C. maritima and decreased during the first 24 h of salt stress and then remained low in A. thaliana. The results clearly showed that C. maritima, in contrast to A. thaliana, can rapidly evolve physiological and antioxidant mechanisms to adapt to salt and manage the oxidative stress. This may explain, at least partially, the difference in salt tolerance between halophytes and glycophytes.