Fabrication of monodispersed Taxol-loaded particles using electrohydrodynamic atomization
Fabrication of monodispersed Taxol-loaded particles using electrohydrodynamic atomization
复制标题
DOI：
10.1016/j.jconrel.2004.10.011
复制
发表时间：
2005-02-02
影响因子：
10.8
通讯作者：
Wang, CH
中科院分区：
文献类型：
--
作者：
Ding, L;Lee, T;Wang, CH
在控制药物释放基质的制备中，单分散的颗粒尺寸通常是首选的，因为它们可以提供更均匀和精确的控制释放轮廓。电流体动力雾化（EHDA） [j]杨建军，杨建军，杨建军，杨建军，电流体雾化技术的研究进展，中国科学院学报（自然科学版）生理学报，2001,31(2):335 - 341。是一种可能产生多分散性非常低的粒子的方法。本文采用EHDA法制备了负载紫杉醇的聚己内酯（PCL）颗粒。努力调查EHDA收率低的原因，并将其提高到80%左右。这是通过增加通风和适当地排出滤纸上颗粒饼上的残余电荷来实现的。采用相位多普勒粒子分析仪（PDPA）检测EHDA的喷雾方式，确定最佳操作条件。用差示扫描量热法（DSC）研究了药物和聚合物基质的均匀性。适度的zeta电位值与激光共聚焦显微照片一起给出了体外细胞摄取数据的可能解释。这些结果证实了相当高的包封效率（EE）和超过1个月的缓释概况。EHDA方法是制备接近单分散药物释放颗粒的一种潜在的合适技术。(C) 2004 Elsevier B.V.版权所有
In the fabrication of controlled drug release matrix, monodispersed particle sizes are usually preferred as they give a more uniform and precisely controlled release profile. Electrohydrodynamic atomization (EHDA) [J.C. Ijsebaert, K.B. Geerse, J.C.M. Marijnissen, JWJ Lammers, P. Zarmen, Electro-Hydrodynamic atomization of drug solutions for inhalation purposes, J. Appl. Physiol. 91(2001), 2735-2741.] is a method that can potentially produce particles with very low polydispersity. In the present work, Taxol-loaded poly-caprolactone (PCL) particles were fabricated using EHDA. Effort was undertaken to investigate the cause of the low yield of EHDA and to improve it to around 80%. This was achieved by increasing the ventilation and properly discharging the residual charges on the particulate cake at the filter paper. A phase Doppler particle analyzer (PDPA) was used to detect the spray modes of EHDA and the optimum operating conditions were determined. With differential scanning calorimetry (DSC), the uniformity of drug and polymer matrix is investigated. Moderate zeta potential values together with laser confocal micrographs give a possible explanation for the in vitro cell uptake data. These results are substantiated by a reasonably high encapsulation efficiency (EE) and sustained release profiles over 1-month period. The EHDA method is shown to be a potentially suitable technique to prepare close to monodispersed drug release particles. (C) 2004 Elsevier B.V. All rights reserved.