Transcriptome analysis in tardigrade species reveals specific molecular pathways for stress adaptations.
Transcriptome analysis in tardigrade species reveals specific molecular pathways for stress adaptations.
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DOI：
10.4137/bbi.s9150
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发表时间：
2012
影响因子：
5.8
通讯作者：
Dandekar T
中科院分区：
文献类型：
--
作者：
Förster F;Beisser D;Grohme MA;Liang C;Mali B;Siegl AM;Engelmann JC;Shkumatov AV;Schokraie E;Müller T;Schnölzer M;Schill RO;Frohme M;Dandekar T
缓步动物具有独特的压力适应能力，使它们能够在极端的寒冷、炎热、辐射和真空中生存。为了研究这一点，使用生物信息学工具对正在进行的缓步动物 Milnesium tardigradum 转录组研究中的编码蛋白簇和通路进行了分析，并与 Hypsibius dujardini 的表达序列标签 (EST) 进行了比较，揭示了参与抵抗极端环境条件的主要通路。 Tardigrade Workbench 上提供 EST 以及软件和数据库更新。我们的分析表明，M. tardigradum 的 RNA 稳定性基序与高等动物已知的典型基序不同。 M. tardigradum 和 H. dujardini 蛋白簇和保守域意味着糖原、糖脂和特定次级代谢的代谢储存途径以及应激反应途径（包括热休克蛋白、bmh2 和特定修复途径）。氧化还原、DNA、应激和蛋白质保护途径与特定的修复能力相辅相成，以实现 M. tardigradum 的强大稳健性。这些途径在其他动物中部分保守，即使在人类细胞中，它们的操纵也可以促进应激适应。然而，抵抗力和修复途径的独特组合使得缓步动物和缓步动物尤其具有高度的抗应激能力。
Tardigrades have unique stress-adaptations that allow them to survive extremes of cold, heat, radiation and vacuum. To study this, encoded protein clusters and pathways from an ongoing transcriptome study on the tardigrade Milnesium tardigradum were analyzed using bioinformatics tools and compared to expressed sequence tags (ESTs) from Hypsibius dujardini, revealing major pathways involved in resistance against extreme environmental conditions. ESTs are available on the Tardigrade Workbench along with software and databank updates. Our analysis reveals that RNA stability motifs for M. tardigradum are different from typical motifs known from higher animals. M. tardigradum and H. dujardini protein clusters and conserved domains imply metabolic storage pathways for glycogen, glycolipids and specific secondary metabolism as well as stress response pathways (including heat shock proteins, bmh2, and specific repair pathways). Redox-, DNA-, stress- and protein protection pathways complement specific repair capabilities to achieve the strong robustness of M. tardigradum. These pathways are partly conserved in other animals and their manipulation could boost stress adaptation even in human cells. However, the unique combination of resistance and repair pathways make tardigrades and M. tardigradum in particular so highly stress resistant.