Snc1p v-SNARE transport to the prospore membrane during yeast sporulation is dependent on endosomal retrieval pathways.
Snc1p v-SNARE transport to the prospore membrane during yeast sporulation is dependent on endosomal retrieval pathways.
复制标题
在酵母孢子形成过程中，Snc1p v-SNARE 向原生孢子膜的运输依赖于内体修复途径。
DOI：
10.1111/j.1600-0854.2007.00606.x
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Engebrecht,Joanne
中科院分区：
文献类型：
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作者：
Morishita,Masayo;Mendonsa,Rima;Wright,Jennifer;Engebrecht,Joanne
囊泡交通对于酿酒酵母的孢子形成至关重要。高尔基体相关逆行蛋白（GARP）束缚复合物是从早期内体和晚期内体到高尔基体的逆行交通所必需的。对孢子形成中 GARP 复合体突变体的分析揭示了减数分裂进展和孢子形成的缺陷。相比之下，逆转录复合体（介导囊泡出芽和晚期内体中的货物选择）或Snx4p（参与早期内体中蛋白质的回收）的失活对孢子形成几乎没有影响。逆转录体 GARP 双突变体在单倍体核周围的原孢子膜 (PSM) 的形成方面存在缺陷。在逆转录体 GARP 双突变体中，携带货物 Dtr1p 的 PSM 前体囊泡被运输到纺锤体极体 (SPB)，在那里启动 PSM 形成。然而，在双突变体中，v-SNARE Snc1p 并未转运至 SPB，这表明 PSM 形成的缺陷是由于未能从内体途径中检索 Snc1p 以及其他蛋白质。总而言之，这些结果表明，通过检索对 PSM 和孢子壁形成重要的分子，从内体逆行运输对于孢子形成至关重要。
Vesicular traffic is essential for sporulation inSaccharomyces cerevisiae. The Golgi‐associated retrograde protein (GARP) tethering complex is required for retrograde traffic from both the early and late endosomes to the Golgi. Analyses of GARP complex mutants in sporulation reveal defects in meiotic progression and spore formation. In contrast, inactivation of the retromer complex, which mediates vesicle budding and cargo selection from the late endosome, or Snx4p, which is involved in retrieval of proteins from the early endosome, has little effect on sporulation. A retromer GARP double mutant is defective in the formation of the prospore membrane (PSM) that surrounds the haploid nuclei. In the retromer GARP double mutant, PSM precursor vesicles carrying the cargo, Dtr1p, are transported to the spindle pole body (SPB), where PSM formation is initiated. However, the v‐SNARE Snc1p is not transported to the SPB in the double mutant, suggesting that the defect in PSM formation is because of the failure to retrieve Snc1p, and perhaps other proteins, from the endosomal pathway. Taken together, these results indicate that retrograde trafficking from the endosome is essential for sporulation by retrieving molecules important for PSM and spore wall formation.