Biological freezing and cryofixation
Biological freezing and cryofixation
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生物冷冻和冷冻固定
DOI：
10.1111/j.1365-2818.1977.tb00042.x
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发表时间：
1977
影响因子：
2
通讯作者：
F. Franks
中科院分区：
文献类型：
--
作者：
F. Franks
冷冻和冷冻固定是实现超微结构和生物保存的常用方法。生物材料中的冻结是复杂的，因为它们具有异质性--水的分布不均匀，各个区域由半透膜隔开。要考虑的过程包括：(1)导致水重新分配的渗透梯度，(2)冰晶的成核和不受控制的生长，(3)成核的水基质的重结晶。
Freezing and freeze fixation are commonly used to achieve ultrastructural and biological preservation. Freezing in biological materials is complex because of their heterogeneous nature—water is unevenly distributed and the various domains are separated by semi‐permeable membranes. Processes to be considered include: (1) osmotic gradients leading to redistribution of water, (2) nucleation and uncontrolled growth of ice crystals, (3) recrystallization of nucleated aqueous substrate.