EVIDENCE FOR A SUBSET OF RHEUMATOID FACTORS THAT CROSS-REACT WITH DNA-HISTONE AND HAVE A DISTINCT CROSS-IDIOTYPE
EVIDENCE FOR A SUBSET OF RHEUMATOID FACTORS THAT CROSS-REACT WITH DNA-HISTONE AND HAVE A DISTINCT CROSS-IDIOTYPE
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DOI：
10.1084/jem.151.6.1514
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发表时间：
1980-01-01
影响因子：
15.3
通讯作者：
JOSLIN, F
中科院分区：
文献类型：
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作者：
AGNELLO, V;ARBETTER, A;JOSLIN, F
证明了[人]单克隆类风湿因子与存在于Ig[免疫球蛋白]G和dna -核蛋白上的抗原的交叉反应性，并有证据表明该抗体的结合位点参与了该反应。DNA-核蛋白上的抗原涉及DNA和组蛋白片段H2A + H2B，对胰蛋白酶敏感。该抗体对IgG的相对结合亲和力似乎大于dna -组蛋白抗原。在多种疾病中发现了类似的多克隆交叉反应性类风湿因子。类风湿关节炎和混合性结缔组织病的发病率较高。在系统性红斑狼疮和特发性冷球蛋白血症患者中没有检测到。对1个代表性的多克隆类风湿因子的研究表明，与单克隆抗体一样，类风湿因子与dna -组蛋白H2A + H2B具有相同的反应性。采用抗原结合抑制方法进行交叉独特型研究，证实与dna组蛋白反应的多克隆和单克隆类风湿因子具有相同的交叉独特型。这种交叉独特型不同于先前在抗- γ单克隆IgM蛋白上显示的交叉独特型。球蛋白的活动。
Cross-reactivity of a [human] monoclonal rheumatoid factor with an antigen present on Ig[immunoglobulin]G and DNA-nucleoprotein was demonstrated, and evidence presented that the combining site of the antibody was involved in the reaction. The antigen on the DNA-nucleoprotein involved DNA and histone fraction H2A + H2B and was trypsin sensitive. The relative binding affinity of the antibody appeared to be greater for IgG than the DNA-histone antigen. Similar polyclonal cross-reactive rheumatoid factors were found in a variety of diseases. A high incidence was found among patients with rheumatoid arthritis and mixed connective tissue disease. None were detected in patients with systemic lupus erythematosus and idiopathic cryoglobulinemia. Studies on 1 representative isolated polyclonal rheumatoid factor demonstrated the same reactivity with DNA-histone H2A + H2B as the monoclonal antibody. Cross-idiotype studies using antigen-binding inhibition methods demonstrated the same cross-idiotype on the polyclonal and the monoclonal rheumatoid factor which reacted with DNA-histone. This cross-idiotype was distinct from the cross-idiotypes previously demonstrated on monoclonal IgM proteins with anti-.gamma.-globulin activity.