Baicalein, a novel apoptotic agent for hepatoma cell lines: A potential medicine for hepatoma
Baicalein, a novel apoptotic agent for hepatoma cell lines: A potential medicine for hepatoma
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DOI：
10.1207/s15327914nc382_19
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发表时间：
2000-01-01
影响因子：
2.9
通讯作者：
Lu, FJ
中科院分区：
文献类型：
--
作者：
Chen, CH;Huang, LLH;Lu, FJ
这项研究证明黄芩素对人类肝癌细胞具有抗癌功效。黄芩素在 Hep G2 和 Hep J2 细胞中的剂量反应表明，黄芩素使活力降低 > 90%。相比之下，黄芩素对对照 Chang 肝细胞的活力只有很小的影响。流式细胞术分析表明黄芩素抑制Hep G2细胞的细胞周期处于S期。此外，黄芩素处理导致线粒体跨膜电位降低并破坏细胞膜的完整性。 TdT 介导的 dUTP-生物素缺口末端标记测定结果表明，在孵育 48 小时后，黄芩素引起 Hep G2 细胞中 DNA 片段的显着增加。这些结果表明黄芩素是一种有效的抗肝癌剂，对非癌细胞的影响最小。黄芩素对 Hep G2 细胞的作用包括抑制细胞周期的 S 期、线粒体功能障碍和启动细胞凋亡。
This study has demonstrated that baicalein has anticancer effectiveness against human hepatoma cells. The dose response of baicalein in Hep G2 and Hep J2 cells indicates that baicalein decreased viability > 90%. In comparison, baicalein had only minimal effects on the viability of control Chang liver cells. Flow cytometric analysis showed that baicalein inhibited the cell cycle of Hep G2 cells in the S phase. In addition, baicalein treatment resulted in a decreased mitochondrial transmembrane potential and damaged the integrity of the cell membrane. The TdT-mediated dUTP-biotin nick end labeling assay results indicated that baicalein elicited a significant increase of DNA fragmentation in Hep G2 cells after incubation for 48 hours. These results indicate that baicalein is an effective antihepatoma agent with minimal influence on noncancer cells. The effects of baicalein on Hep G2 cells include inhibition of the S phase of the cell cycle, dysfunction of mitochondria, and initiation of apoptosis.