Determination of skin wound age by using cytokines as potential markers
Determination of skin wound age by using cytokines as potential markers
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DOI：
10.1016/j.jflm.2016.08.011
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发表时间：
2016-11-01
影响因子：
1.5
通讯作者：
Ince, Imran
中科院分区：
文献类型：
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作者：
Birincioglu, Ismail;Akbaba, Murat;Ince, Imran
白细胞介素-1 β (IL-1 β)、IL-6、肿瘤坏死因子- α (tnf - α)和表皮生长因子（EGF）在伤口愈合过程中发挥重要作用。在本研究中，人体伤口标本（n = 50）是从枪支伤害、尖锐物体穿透性创伤和钝性创伤死亡病例中收集的，法医尸检确定了已知的受伤和死亡时间。从受伤皮肤部位获得全层组织标本，并从同一个人获得相同大小的完整组织作为对照。根据Bradford法对每个标本进行酶联免疫吸附试验（ELISA）蛋白测定，并提供单个蛋白的测定结果。各创面组IL-1 β水平均无统计学意义，且均未显著高于对照组。而IL-6在创面小于30min组和创面大于18h组均呈双相分布，且差异有统计学意义。IL-6在所有创面组均高于对照组。除创面2-4 h外，所有组的tnf - α在创面30分钟内均有统计学意义的升高，并保持在较高水平。另一方面，除2 ~ 4 h和18 h以上创面组外，其余各组EGF均较高，但差异无统计学意义。我们的结果表明，特别是IL-6和tnf - α可能用作早期标志物。我们认为IL-1b和EGF应该在进一步的研究中进行更广泛的评估。(C) 2016爱思唯尔有限公司和法医和法律医学学院。版权所有。
Interleukin-1 beta ( IL-1 beta), IL-6, tumour necrosis factor-alpha (TNF-alpha) and epidermal growth factor (EGF) play important roles in the wound healing process. In the present study, human wound specimens (n = 50) were collected from cases of death due to injuries from firearms, penetrating trauma by sharp objects and blunt trauma with a known time of injury and death identified by forensic autopsy. Full-thickness tissue specimens were obtained from injured skin sites, and equally sized intact tissues obtained from the same person were used as controls. Protein determination was performed using ELISA according to the Bradford method for each specimen, and results were provided for individual proteins. IL-1 beta levels did not reach statistical significance in any of the wound groups and were not markedly higher than those in the control group. However, IL-6 showed a biphasic pattern and reached statistical significance in the group with wounds less than 30 min old and in the group with wounds more than 18 h old. IL-6 was consistently higher in all wound groups than in the control group. TNF-alpha showed a statistically significant increase within the first 30 min and remained at a high level in all groups except for those with wounds 2-4 h old. On the other hand, EGF was high in all groups excluding those with wounds 2-4 h old and more than 18 h old, but statistical significance was not reached. Our results suggest that IL-6 and TNF-alpha in particular may be used as early-phase markers. We believe that IL-1b and EGF should be more extensively evaluated in further studies. (C) 2016 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.