PROMOTION OF MUSCLE REGENERATION IN THE TOAD (BUFO-VIRIDIS) GASTROCNEMIUS-MUSCLE BY LOW-ENERGY LASER IRRADIATION
PROMOTION OF MUSCLE REGENERATION IN THE TOAD (BUFO-VIRIDIS) GASTROCNEMIUS-MUSCLE BY LOW-ENERGY LASER IRRADIATION
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DOI：
10.1002/ar.1092350306
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发表时间：
1993-03-01
影响因子：
--
通讯作者：
ORON, U
中科院分区：
文献类型：
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作者：
BIBIKOVA, A;ORON, U
采用定量组织学和形态计量学方法研究低能激光（He-Ne）照射对蟾蜍（Bufo viridis）腓肠肌冷损伤后骨骼肌再生过程的影响。从受伤后第四天开始，每隔一天对实验蟾蜍的受伤区域进行五次直接氦氖激光（632.8 nm 波长）照射（6.0 mW，2.3 分钟）。将如上所述受伤并接受红光照射的肌肉作为对照组。在受伤后 9、14 和 30 天对受伤区域的组织学切片进行形态测定分析。损伤后 9 天，单核细胞占损伤总面积的 69.3% +/-16.8%。此后，激光照射肌肉中的体积分数（总损伤区域的百分比）逐渐下降，但比对照组下降得更快。肌肉再生第 9 天时，激光照射肌肉中肌管的体积分数 (7.0% +/- 2.2%) 显着高于对照肌肉 (1.2% +/- 0.4%)。在肌肉再生第 9 天和第 14 天，激光照射肌肉中的年轻肌纤维分别占受伤区域的 15.5% +/- 7.9% 和 65.0% +/- 9.5%，而在对照肌肉中，这些结构在第 9 天时并不明显，在受伤后 14 天时仅占受伤区域的 5.3% +/- 2.9%。 30天的肌肉再生中，年轻肌纤维的体积分数进一步增加，占受伤区域的75.7%+/-13.2%，而在激光照射的肌肉中，大部分受伤区域充满了成熟的肌纤维。结果表明，氦氖激光照射在再生过程中显着促进蟾蜍腓肠肌冷损伤后损伤区肌肉的成熟。
The effect of low-energy laser (He-Ne) irradiation on the process of skeletal muscle regeneration after cold injury to the gastrocnemius muscle of the toad (Bufo viridis) was studied using quantitative histological and morphometric methods. The injured zones in the experimental toads were subjected to five direct He-Ne laser (632.8 nm wavelength) irradiations (6.0 mW for 2.3 min) every alternate day starting on the fourth day postinjury. Muscles that were injured as above, and subjected to red-light irradiation, served as a control group. Morphometric analysis was performed on histological sections of injured areas at 9, 14, and 30 days postinjury. At 9 days postinjury, mononucleated cells populated 69.3% +/-16.8% of the total area of injury. Thereafter, their volume fraction (percent of total injured zone) decreased gradually but more rapidly in the laser-irradiated muscle than in the control. The volume fraction of the myotubes in the laser-irradiated muscles at 9 days of muscle regeneration was significantly higher (7.0% +/- 2.2%) than in the control muscle (1.2% +/- 0.4%). Young myofibers in the laser-irradiated muscles populated 15.5% +/- 7.9% and 65.0% +/- 9.5% of the injured area at 9 and 14 days of muscle regeneration, respectively, while in control muscles these structures were not evident at 9 days and made up only 5.3% +/- 2.9% of the traumatized area at 14 days postinjury. The volume fraction of the young myofibers further increased by 30 days of muscle regeneration making up 75.7% +/- 13.2% of the traumatized area, while in the laser-irradiated muscles most of the injured zone was filled with mature muscle fibers. It is concluded that He-Ne laser irradiation during the regeneration process markedly promotes muscle maturation in the injured zone following cold injury to the toad gastrocnemius muscle.