Dynamics of bacterial community development in the reef coral Acropora muricata following experimental antibiotic treatment
Dynamics of bacterial community development in the reef coral Acropora muricata following experimental antibiotic treatment
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DOI：
10.1007/s00338-011-0800-0
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发表时间：
2011-07
期刊：
影响因子：
3.5
通讯作者：
M. Sweet;A. Cróquer;J. Bythell
中科院分区：
文献类型：
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作者：
M. Sweet;A. Cróquer;J. Bythell
利用基于16S rRNA基因的技术监测与珊瑚acropora muricata（=formosa）相关的细菌群落的发展，并在使用抗生素环丙沙星对现有微生物群落进行实验修饰后，随着时间的推移进行丰度计数。处理后细菌丰度降低了约99%，细菌群落结构发生了显著变化。在重新部署到自然环境后，一些细菌在几小时内就定居并重新生长，包括在自然微生物群中不存在或丰度低的核糖体类型。然而，细菌群落的完全恢复需要超过96 h的时间，这表明水体中细菌的沉降和生长相对缓慢，表明自然群落的周转也同样缓慢。早期发展的群落主要是来自天然微生物群的耐抗生素细菌，这些细菌在没有天然竞争者的情况下存活下来并增殖，但也包括一些从水柱定植的非常驻核型。几乎所有这些机会主义者在处理后96小时内都被显著减少或消除，这表明天然细菌群落具有很高的恢复力。潜在的病原体，包括梭菌。，在珊瑚中以低丰度栖息，只有当天然微生物群受到处理的干扰时才变得普遍。健康的珊瑚相关微生物群似乎受到微生物相互作用的强烈控制。
Development of the bacterial community associated with the coralAcropora muricata(=formosa) was monitored using 16S rRNA gene-based techniques and abundance counts over time following experimental modification of the existing microbial community using the antibiotic ciprofloxacin. Abundance of bacteria was reduced >99% by the treatment, resulting in significant changes in bacterial community structure. Following redeployment to their natural environment, some settlement and re-growth of bacteria took place within a few hours, including ribosomal types that were not present, or in low abundance, in the natural microbiota. However, complete recovery of the bacterial community required longer than 96 h, which indicates a relatively slow settlement and growth of bacteria from the water column and suggests that turnover of the natural community is similarly slow. The early developing community was dominated by antibiotic-resistant bacteria from the natural microbiota that survived the treatment and proliferated in the absence of natural competitors, but also included some non-resident ribotypes colonizing from the water column. Almost, all these opportunists were significantly reduced or eliminated within 96 h after treatment, demonstrating a high resilience in the natural bacterial community. Potential pathogens, including aClostridiumsp., inhabited the coral at low abundances, only becoming prevalent when the natural microbiota was disturbed by the treatment. The healthy coral-associated microbiota appears to be strongly controlled by microbial interactions.