Importance of rare taxa for bacterial diversity in the rhizosphere of Bt- and conventional maize varieties
Importance of rare taxa for bacterial diversity in the rhizosphere of Bt- and conventional maize varieties
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DOI：
10.1038/ismej.2012.77
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发表时间：
2013-01-01
期刊：
影响因子：
11
通讯作者：
Tebbe, Christoph C.
中科院分区：
文献类型：
--
作者：
Dohrmann, Anja B.;Kueting, Meike;Tebbe, Christoph C.
核糖体16 S rRNA基因焦磷酸测序用于探索表达苏云金芽孢杆菌的三种杀虫重组Cry蛋白的转基因（GM）Bt玉米杂交种MON 89034 x MON 88017是否会改变根际细菌群落。对大田种植的Bt玉米和3个常规玉米品种的细根以及Bt玉米的粗根进行了分析。共获得547 000个序列。文库覆盖率在门为100%，属为99.8%。虽然基于相对丰度的聚类分析表明，在较高的分类等级没有差异，属的丰度指向品种的具体差异。基于属的聚类仅依赖于49个最优势的属，而其余的461个稀有属遵循不同的选择。共有91个属对不同的根环境有显著的响应。作为焦磷酸测序的益处，鉴定了79个响应属，这些属由于其稀有性而可能被常规克隆测序方法忽略。有没有迹象表明细菌的BT-玉米根际的变化超出了传统品种之间的差异。B。苏云金杆菌样Cry类型以低丰度（细菌的0.1%）存在，表明根际中可能存在天然Cry蛋白。虽然一些属表明潜在的植物病原细菌在根际，其丰度之间的常规品种和Bt-玉米没有显着差异。这项研究以前所未有的灵敏度表明，转基因玉米根际细菌群落对根组织中三种杀虫蛋白的存在没有异常反应。The ISME Journal（2013）7，37-49; doi：10.1038/ismej.2012.77; 2012年7月12日在线发表
Ribosomal 16S rRNA gene pyrosequencing was used to explore whether the genetically modified (GM) Bt-maize hybrid MON 89034 x MON 88017, expressing three insecticidal recombinant Cry proteins of Bacillus thuringiensis, would alter the rhizosphere bacterial community. Fine roots of field cultivated Bt-maize and three conventional maize varieties were analyzed together with coarse roots of the Bt-maize. A total of 547 000 sequences were obtained. Library coverage was 100% at the phylum and 99.8% at the genus rank. Although cluster analyses based on relative abundances indicated no differences at higher taxonomic ranks, genera abundances pointed to variety specific differences. Genera-based clustering depended solely on the 49 most dominant genera while the remaining 461 rare genera followed a different selection. A total of 91 genera responded significantly to the different root environments. As a benefit of pyrosequencing, 79 responsive genera were identified that might have been overlooked with conventional cloning sequencing approaches owing to their rareness. There was no indication of bacterial alterations in the rhizosphere of the Bt-maize beyond differences found between conventional varieties. B. thuringiensis-like phylotypes were present at low abundance (0.1% of Bacteria) suggesting possible occurrence of natural Cry proteins in the rhizospheres. Although some genera indicated potential phytopathogenic bacteria in the rhizosphere, their abundances were not significantly different between conventional varieties and Bt-maize. With an unprecedented sensitivity this study indicates that the rhizosphere bacterial community of a GM maize did not respond abnormally to the presence of three insecticidal proteins in the root tissue. The ISME Journal (2013) 7, 37-49; doi:10.1038/ismej.2012.77; published online 12 July 2012