Cancer-associated orthotopic myofibroblasts stimulates the motility of gastric carcinoma cells
Cancer-associated orthotopic myofibroblasts stimulates the motility of gastric carcinoma cells
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DOI：
10.1111/j.1349-7006.2012.02209.x
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发表时间：
2012-04-01
期刊：
影响因子：
5.7
通讯作者：
Hirakawa, Kosei
中科院分区：
文献类型：
--
作者：
Fuyuhiro, Yuhiko;Yashiro, Masakazu;Hirakawa, Kosei
肿瘤进展被认为是癌细胞和周围间质细胞之间相互干扰的产物。癌相关的原位肌成纤维细胞可能与胃癌的进展有关。为了了解原位肌成纤维细胞的意义，我们检测了癌症相关的原位肌成纤维细胞对胃癌细胞恶性表型的影响。选用3株人胃癌细胞株(OCUM-2MD3、OCUM-12、MKN-45)和4株人胃成纤维细胞株(癌相关原位成纤维细胞[CAF]-29、CAF-33、正常原位成纤维细胞[NF]-29、NF-33)。从肿瘤胃壁建立了癌相关的原位成纤维细胞系CAF-29和CAF-33，从非肿瘤胃壁建立了正常的原位成纤维细胞系NF-29和NF-33。α-平滑肌肌动蛋白阳性的成纤维细胞称为肌成纤维细胞。我们通过伤口愈合试验、侵袭试验和RT-PCR检测了癌相关原位肌成纤维细胞对胃癌细胞侵袭性的影响。CAF-29(33%)和CAF-33(46%)成肌细胞比例差异有统计学意义(P
Tumor progression has been recognized as the product of evolving crosstalk between cancer cells and the surrounding stromal cells. Cancer-associated orthotopic myofibroblasts may be linked to the progression of gastric carcinomas. To understand the significance of orthotopic myofibroblasts, we examined the effects of cancer-associated orthotopic myofibroblasts on the malignant phenotype of gastric cancer cells. Three human gastric cancer cell lines (OCUM-2MD3, OCUM-12, MKN-45) and four human gastric fibroblast cell lines (cancer-associated orthotopic fibroblast [CaF]-29, CaF-33, normal orthotopic fibroblast [NF]-29, NF-33) were used. The cancer-associated orthotopic fibroblast cell lines CaF-29 and CaF-33 were established from a tumoral gastric wall, and normal orthotopic fibroblast NF-29 and NF-33 were established from a non-tumoral gastric wall. Fibroblasts that were a-smooth muscle actin-positive were defined as myofibroblasts. We examined the effects of cancer-associated orthotopic myofibroblasts on the aggressiveness of gastric cancer cells by wound-healing assay, invasion assay, and RT-PCR. The ratios of myofibroblasts in CaF-29 (33%) and CaF-33 (46%) were significantly (P similar to