EST-based identification of genes expressed in brain and spinal cord of Gekko japonicus, a species demonstrating intrinsic capacity of spinal cord regeneration
EST-based identification of genes expressed in brain and spinal cord of Gekko japonicus, a species demonstrating intrinsic capacity of spinal cord regeneration
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基于 EST 鉴定壁虎大脑和脊髓中表达的基因，该物种表现出脊髓再生的内在能力
DOI：
10.1385/jmn:29:1:21
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发表时间：
2006-01-01
影响因子：
3.1
通讯作者：
Gu, Xiaosong
中科院分区：
文献类型：
--
作者：
Liu, Yan;Ding, Fei;Gu, Xiaosong
在本研究中，构建了壁虎脑和脊髓cDNA文库，并从4108个随机选择的克隆中确定了2349个独特的表达序列标签（EST），由494个簇和1855个单序列组成。在这些序列中，54.8% (1287/2349) EST 被鉴定为已知基因，45.2% (1062/2349) 被鉴定为未知基因。已确定的 EST 根据其功能分类进行分类；管家基因和神经系统相关基因在文库中都有很好的体现。还鉴定了来自该文库的包含完整开放阅读框（ORF）的克隆；迄今为止已获得303个克隆。最后，选择与内皮分化相关因子1（EDF-1）和髓磷脂相关糖蛋白（MAG）前体具有序列同源性的两个克隆，并通过RT-PCR分析它们在正常和再生脊髓中的表达模式。两者均在脊髓中表达，断尾后表达量显着下降。这项研究提供了壁虎中枢神经系统基因表达谱的初步视图，它可能加速该生物体基因发现和功能分析的进程，这证明了脊髓再生的内在能力。
In the present study, a brain and spinal cord cDNA library of Gekko japonicus was constructed, and 2349 unique expressed sequence tags (ESTs), composed of 494 clusters and 1855 singletons, were determined from 4108 randomly selected clones. Of these sequences, 54.8% (1287/2349) ESTs are identified as known genes and 45.2% (1062/2349), as unknown genes. The identified ESTs were classified with respect to their functional categorization; both the housekeeping genes and the nervous system-related genes were well represented in the library. The complete open reading frame (ORF)-containing clones derived from the library were identified as well; 303 clones were obtained to date. Finally, two clones with sequence homology to endothelial differentiation-related factor 1 (EDF-1) and to myelin-associated glycoprotein (MAG) precursor were selected, and their expression patterns in normal and regenerating spinal cord were analyzed by RT-PCR. Both of them were expressed in the spinal cord, and the expression level decreased significantly after tail amputation. This study provides a preliminary view of the gene expression profiles of the central nervous system in gecko, and it might accelerate the process of gene discovery and function analysis in this organism, which demonstrates an intrinsic capacity of spinal cord regeneration.