Salmonella enterica Serovar Enteritidis Genes Induced during Oviduct Colonization and Egg Contamination in Laying Hens
Salmonella enterica Serovar Enteritidis Genes Induced during Oviduct Colonization and Egg Contamination in Laying Hens
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DOI：
10.1128/aem.01087-08
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发表时间：
2008-11-01
影响因子：
4.4
通讯作者：
Van Immerseel, F.
中科院分区：
文献类型：
--
作者：
Gantois, I.;Ducatelle, R.;Van Immerseel, F.
肠炎沙门氏菌是世界范围内与沙门氏菌病有关的主要血清型，部分原因是它能够污染鸡蛋的内部内容物。研究表明，肠炎沙门氏菌对禽类生殖道有一种特殊的嗜性，并具有在输卵管和卵巢中持续存在的能力。允许肠炎沙门氏菌菌株污染卵子的因素可能是与输卵管组织的特定相互作用，导致持续的输卵管定植。采用启动子-陷阱的体内表达技术，筛选肠炎沙门氏菌在输卵管定植和卵子污染过程中表达的基因。从输卵管组织和产卵中分离到25个含有体内诱导启动子的克隆。在25个无性系中，有7个是从输卵管和卵中分离出来的。对克隆的启动子进行DNA测序表明，与氨基酸和核酸代谢、运动性、细胞壁完整性和应激反应相关的基因在蛋鸡的生殖道组织中高度表达。
Salmonella enterica serovar Enteritidis is the predominant serovar associated with salmonellosis worldwide, which is in part due to its ability to contaminate the internal contents of the hen's egg. It has been shown that S. enterica serovar Enteritidis has an unusual tropism for the avian reproductive tract and an ability to persist in the oviduct and ovary. Factors allowing S. enterica serovar Enteritidis strains to contaminate eggs could be a specific interaction with the oviduct tissue, leading to persisting oviduct colonization. In vivo expression technology, a promoter-trap strategy, was used to identify genes expressed during oviduct colonization and egg contamination with S. enterica serovar Enteritidis. A total of 25 clones with in vivo-induced promoters were isolated from the oviduct tissue and from laid eggs. Among the 25 clones, 7 were isolated from both the oviducts and the eggs. DNA sequencing of the cloned promoters revealed that genes involved in amino acid and nucleic acid metabolism, motility, cell wall integrity, and stress responses were highly expressed in the reproductive tract tissues of laying hens.