Identification and expression of gene transcripts generated during an anti-parasitic response in Biomphalaria glabrata
Identification and expression of gene transcripts generated during an anti-parasitic response in Biomphalaria glabrata
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DOI：
10.1016/j.dci.2006.10.001
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Coustau, Christine
中科院分区：
文献类型：
--
作者：
Guillou, Francois;Mitta, Guillaume;Coustau, Christine
为了进一步了解腹足类抗寄生虫免疫反应的分子基础，我们研究了血细胞包裹过程中调控的光滑双脐螺转录本。使用的蜗牛菌株，是抗寄生虫棘口线虫caproni，我们进行抑制性消减杂交（SSH）构建的cDNA文库的转录本更丰富地表达在未暴露或寄生虫暴露的蜗牛。经过序列分析和定量PCR分析的表达，我们确定了10个候选人特别感兴趣。它们属于不同的功能组，如解毒酶（GST，SOD），抗菌蛋白（LBI/BPI），蛋白酶抑制剂（半胱氨酸蛋白酶抑制剂），钙结合蛋白，或C型凝集素。原位杂交（ISH）分析表明，一个过表达的半胱氨酸蛋白酶抑制剂样候选人是专门表达在血细胞参与寄生虫封装或聚集在感染部位。另外两个候选者（C型凝集素和LBP/BPI）在蛋白腺中表达，进一步支持了该器官在免疫和/或宿主-寄生虫相互作用中的作用。(c)2006爱思唯尔有限公司保留所有权利。
In order to gain further insights into the molecular basis of gastropod anti-parasite immune responses, we investigated transcripts of Biomphalaria glabrata regulated during hemocytic encapsulation. Using a snail strain that is resistant to the parasite Echinostoma caproni, we performed suppression subtractive hybridization (SSH) to construct cDNA libraries of transcripts more abundantly expressed in unexposed or parasite-exposed snails. After sequence analysis and quantitative PCR analysis of expression, we identified 10 candidates of particular interest. They belonged to various functional groups such as detoxification enzymes (GST, SOD), antimicrobial proteins (LBI/BPI), protease inhibitors (cystatins), calcium-binding proteins, or C-type lectins. In situ hybridization (ISH) analysis revealed that one overexpressed cystatin-like candidate is specifically expressed in hemocytes participating in parasite encapsulation or aggregating at the site of infection. Two other candidates (C-type lectin and a LBP/BPI) were expressed in the albumen gland, further supporting the role of this organ in immunity and/or host-parasite interaction. (c) 2006 Elsevier Ltd. All rights reserved.