Microsatellite DNA Polymorphism to Reveal Genetic Divergence in Ayu, Plecoglossus altivelis
Microsatellite DNA Polymorphism to Reveal Genetic Divergence in Ayu, Plecoglossus altivelis
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微卫星 DNA 多态性揭示香鱼、香鱼的遗传差异
DOI：
10.2331/fishsci.65.507
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发表时间：
1999
影响因子：
1.9
通讯作者：
N. Taniguchi
中科院分区：
文献类型：
--
作者：
M. Takagi;Eijiro Shoji;N. Taniguchi
Six (CA)n and one (CT)n(CA)n microsatellite loci were isolated from a size-selected genomic libra ry of ayu Plecoglossus altivelis altivelis. Primers for PCR amplification were designed for the microsatellite loci and the loci characterized by screening polymorphisms in the amphidromous form of ayu. All loci displayed a high degree of length polymorphism, as observed in the total number of alleles per locus (2-21), and a high degree of heterozygosity ranging from 0.414-1.000. Distinct differences were observed between amphidromous and landlocked populations of ayu, in the frequency distribu tions of the alleles. The primers developed for ayu Plecoglossus a. altivelis were also tested for their abil ity to amplify homologous sequences in endangered subspecies of Ryukyu-ayu, Plecoglossus altivelis ryukyuensis. Genetic variability was obviously low in both average number of alleles per locus and average heterozygosities. These microsatellite loci show great potential as indicators for genetic variabil ity and divergence among populations of ayu species.
