PRODUCTION OF ENKEPHALIN IN TOBACCO PROTOPLASTS USING TOBACCO MOSAIC-VIRUS RNA VECTOR
PRODUCTION OF ENKEPHALIN IN TOBACCO PROTOPLASTS USING TOBACCO MOSAIC-VIRUS RNA VECTOR
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DOI：
10.1016/0014-5793(90)81121-4
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发表时间：
1990-08-20
期刊：
影响因子：
3.5
通讯作者：
OKADA, Y
中科院分区：
文献类型：
--
作者：
TAKAMATSU, N;WATANABE, Y;OKADA, Y
为了验证将外源基因与烟草花叶病毒（TMV）外壳蛋白（CP）融合表达的有效性，我们利用TMV RNA体外转录系统构建了ENK RNA。ENK RNA与TMV RNA的不同之处仅在于ENK RNA携带编码Leu‐enkephalin（Tyr‐Gly‐Gly‐Phe‐Leu）（Enk）的额外序列，在CP基因的终止密码子之前具有前一个框内甲硫氨酸。在接种ENK RNA的原生质体中，CP+Enk融合蛋白作为主要蛋白积累。
To examine the validity of the strategy to express a foreign gene as a fusion protein with the coat protein (CP) of tobacco mosaic virus (TMV), we have constructed ENK RNA by using an in vitro transcription system of TMV RNA. ENK RNA differs from TMV RNA only in that ENK RNA carries an additional sequence coding for Leu‐enkephalin (Tyr‐Gly‐Gly‐Phe‐Leu) (Enk) with a preceding in‐frame methionine just before the termination codon of CP gene. In protoplasts inoculated with ENK RNA, CP+Enk fusion protein accumulated as the major protein.