External validation of blood eosinophils, FENO and serum periostin as surrogates for sputum eosinophils in asthma
External validation of blood eosinophils, FENO and serum periostin as surrogates for sputum eosinophils in asthma
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DOI：
10.1136/thoraxjnl-2014-205634
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发表时间：
2015-02-01
期刊：
影响因子：
10
通讯作者：
Sterk, P. J.
中科院分区：
文献类型：
--
作者：
Wagener, A. H.;de Nijs, S. B.;Sterk, P. J.
背景：监测哮喘患者痰嗜酸性粒细胞可预测哮喘急性发作并改善对哮喘的治疗。到目前为止，血嗜酸性粒细胞和FENO在预测嗜酸性粒细胞气道炎症方面显示出相互矛盾的结果。最近，血清periostin被提出作为一种新的生物标志物嗜酸性inflammation.Objectives量化的相互关系的血液嗜酸性粒细胞，FENO，血清periostin痰嗜酸性粒细胞的外部验证在两个独立的coholts在不同的严重程度asthen.Methods第一个队列包括110例轻度至中度哮喘（外部验证队列）。重复队列包括37例中度至重度哮喘患者。对两个队列进行了横断面评价。诱导痰液用于评估嗜酸性粒细胞。同时，评估血液嗜酸性粒细胞计数、血清骨膜蛋白浓度和FENO。采用受试者工作特征曲线下面积（ROC AUC）计算这些指标对嗜酸粒细胞性哮喘（痰液嗜酸粒细胞<3%）的诊断准确性。
Background Monitoring sputum eosinophils in asthma predicts exacerbations and improves management of asthma. Thus far, blood eosinophils and FENO show contradictory results in predicting eosinophilic airway inflammation. More recently, serum periostin was proposed as a novel biomarker for eosinophilic inflammation.Objectives Quantifying the mutual relationships of blood eosinophils, FENO, and serum periostin with sputum eosinophils by external validation in two independent cohorts across various severities of asthma.Methods The first cohort consisted of 110 patients with mild to moderate asthma (external validation cohort). The replication cohort consisted of 37 patients with moderate to severe asthma. Both cohorts were evaluated cross-sectionally. Sputum was induced for the assessment of eosinophils. In parallel, blood eosinophil counts, serum periostin concentrations and FENO were assessed. The diagnostic accuracy of these markers to identify eosinophilic asthma (sputum eosinophils 3%) was calculated using receiver operating characteristics area under the curve (ROC AUC).Results In the external validation cohort, ROC AUC for blood eosinophils was 89% (p