Profiling circulating microRNAs in serum of Fasciola gigantica-infected buffalo
Profiling circulating microRNAs in serum of Fasciola gigantica-infected buffalo
复制标题
巨片形吸虫感染水牛血清中循环 microRNA 的分析
DOI：
10.1016/j.molbiopara.2019.111201
复制
发表时间：
2019-09-01
影响因子：
1.5
通讯作者：
Guo, Aijiang
中科院分区：
文献类型：
--
作者：
Guo, Xiaola;Guo, Aijiang
循环miRNA在血清和血浆中稳定存在，可作为一类新的蠕虫感染诊断生物标志物。肝片形吸虫是肝片形吸虫病的病原体，主要寄生于人和反刍动物的肝脏，特别是牛、山羊和绵羊的肝脏。在这项研究中，共有121种宿主循环miRNA差异表达（2 >=倍数变化，p < 0.05），其中44种miRNA上调，77种miRNA显著下调。qRT-PCR结果显示，在F. 21- 5 p和bta-miR-23 a的表达水平逐渐升高，而bta-miR-125 a的表达水平逐渐降低，与测序结果一致。感染时间点。四个F在F.t.血清中鉴定出三种已知的miRNA（fgi-miR-87、fgi-miR-71和fgi-miR-124）和一种新的miRNA（新的miR-1）。被南极洲感染的水牛进一步的分析表明，两种寄生虫来源的miRNAs（fgi-miR-87和fgi-miR-71）在F.被南极洲感染的水牛这些发现将有助于理解循环miRNA在宿主-寄生虫相互作用中的作用，并加强血清miRNA作为F.南极洲
Circulating miRNAs are stably existed in serum and plasma and can serve as a novel class of biomarkers for the diagnosis of helminthic infection. Fasciola gigantica, the causative agents of fascioliasis, live in the liver of in humans and ruminants, especially cattle, goat and sheep. In this study, a total of 121 host circulating miRNAs were differentially expressed (2 >= fold change, p < 0.05), of which 44 miRNAs were up-regulated and 77 miRNAs were significantly down-regulated. Consistent with the sequencing data, qRT-PCR results showed that the expression levels of bta-miR-21-5p and bta-miR-23a were elevated gradually and bta-miR-125a was decreased gradually at the F. gigantica infection time points. Four F. gigantica-specific miRNAs, including three known miRNAs (fgi-miR-87, fgi-miR-71, and fgi-miR-124), and one novel miRNA (novel miR-1) were identified in the sera of F. gigantica-infected buffaloes. Further analyses demonstrated that two parasite-derived miRNAs (fgi-miR-87 and fgi-miR-71) were specifically detected in sera of F. gigantica-infected buffaloes. These findings will be helpful to understand the roles of circulating miRNAs in host-parasite interaction and to potentiate serum miRNAs as diagnostic targets for F. gigantica.