Duck-origin H5N6 avian influenza viruses induce different pathogenic and inflammatory effects in mice
Duck-origin H5N6 avian influenza viruses induce different pathogenic and inflammatory effects in mice
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鸭源 H5N6 禽流感病毒对小鼠产生不同的致病和炎症作用。
DOI：
10.1111/tbed.13956
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发表时间：
2021
影响因子：
4.3
通讯作者：
Xu Chenggang
中科院分区：
文献类型：
--
作者：
Xiang Bin;Song Jie;Chen Libin;Liang Jianpeng;Li Xin;Yu Deshui;Lin Qiuyan;Liao Ming;Ren Tao;Xu Chenggang
自2013年以来，H5N6高致病性禽流感病毒给家禽业造成了相当大的经济损失，并造成24例实验室确认的人间病例。在这项研究中，我们从2018年12月至2019年4月中国南方广东省的健康鸭子中分离出9种（B1-B9） H5N6病毒。系统发育分析显示，该病毒B1、B2、B3、B4、B5、B7、B8和B9聚类为G1.1基因型，与2017年和2018年华南地区分离的H5N6病毒序列具有高度相似性。B6聚为G1.1.9基因型。B6的血凝素（HA）、神经氨酸酶（NA）和非结构蛋白（NS）基因片段与人类H5N6分离株密切相关，其他基因组片段与华南水禽H5N6分离株密切相关。与B7相比，B6在小鼠中具有更高的致病性和更强的炎症反应。B6携带全长PB1‐F2蛋白（90 aa），其余携带11个氨基酸的C末端截断PB1‐F2。与B7相比，PB1‐F2蛋白可能会增加B6的毒力。我们的研究结果为H5N6病毒在哺乳动物中的致病机制提供了见解，并强调了继续监测鸭子中流行的H5N6病毒的必要性。
Since 2013, H5N6 highly pathogenic avian influenza viruses have caused considerable economic losses in the poultry industry and have caused 24 laboratory‐confirmed human cases. In this study, we isolated nine (B1–B9) H5N6 viruses from healthy ducks in Guangdong Province, Southern China from December 2018 to April 2019. Phylogenetic analysis revealed that B1, B2, B3, B4, B5, B7, B8, and B9 clustered into the G1.1 genotype and shared high sequence similarity with human H5N6 isolates from Southern China in 2017 and 2018. Meanwhile, B6 clustered into the G1.1.9 genotype. The hemagglutinin (HA), neuraminidase (NA) and nonstructural protein (NS) gene segments of B6 were closely related to the human H5N6 isolates, while the other genomic segments were closely related to H5N6 viruses isolated from waterfowl in Southern China. Compared to B7, B6 had higher pathogenicity and induced stronger inflammatory responses in mice. B6 carried a full‐length PB1‐F2 protein (90 aa), while the rest carried an 11‐amino acid C‐terminal‐truncated PB1‐F2. The PB1‐F2 protein may increase the virulence of B6 compared to that of B7. Our findings provide insight into the pathogenic mechanisms of H5N6 viruses in mammals and emphasize the need for continued surveillance of circulating H5N6 viruses in ducks.